Based on the development of automatic devices and rapid assay methods, various high-throughput screening (HTS) strategies have been established for improving the performance of industrial microorganisms. We discuss the most significant factors that can improve HTS efficiency, including the construction of screening libraries with high diversity and the use of new detection methods to expand the search range and highlight target compounds. We also summarize applications of HTS for enhancing the performance of industrial microorganisms. Current challenges and potential improvements to HTS in industrial biotechnology are discussed in the context of rapid developments in synthetic biology, nanotechnology, and artificial intelligence. Rational integration will be an important driving force for constructing more efficient industrial microorganisms with wider applications in biotechnology.

基于自动设备和快速测定方法的发展，已经建立了各种用于提高工业微生物性能的高通量筛选（HTS）策略。我们讨论了可以提高HTS效率的最重要因素，包括构建具有高度多样性的筛选库以及使用新的检测方法来扩大搜索范围并突出显示目标化合物。我们还总结了HTS在增强工业微生物性能方面的应用。在合成生物学，纳米技术和人工智能的快速发展背景下，讨论了工业生物技术中HTS的当前挑战和潜在改进。