Ultra-structural analysis of human spermatozoa by aperture scanning near-field optical microscopy.,Journal of Biophotonics

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Ultra-structural analysis of human spermatozoa by aperture scanning near-field optical microscopy.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-02-13 , DOI: 10.1002/jbio.201960093
Barbara Troian ₁ , Rita Boscolo ₂ , Giuseppe Ricci _{2,

3} , Marco Lazzarino ₄ , Gabriella Zito ₂ , Stefano Prato ₁ , Laura Andolfi ₄

Affiliation

Scanning near‐field optical microscopy (SNOM) represents a potential candidate for investigation of ultrastructure in human spermatozoa. It is a noninvasive optical technique that offers two main advantages: minimal sample preparation and simultaneous topographical and optical images acquisition with a spatial resolution beyond the diffraction limit. This enables the combination of surface characterization and information from the inner cellular organization in a single acquisition providing an immediate and comprehensive analysis of the cellular portions. In this work spermatozoa are immobilized on poly‐L‐lysine coated coverslips, fixed according to a standard protocol and imaged by aperture‐SNOM in air. In the SNOM images, all peculiar sperm portions show well‐resolved optical features, which exhibit good similarities with the structures revealed in transmission electron microscopy images, as compared with literature data. The optical features of anomalous spermatozoa are clearly different as respect with those observed for healthy ones. This analysis reveals the potentialities of SNOM and opens to its application to high‐resolution analysis of sperm morphological alterations, which might be relevant in reproductive medicine.

中文翻译：

通过孔径扫描近场光学显微镜对人精子的超微结构分析。

扫描近场光学显微镜（SNOM）代表了人类精子超微结构研究的潜在候选人。它是一种非侵入性光学技术，具有两个主要优点：最少的样品制备以及同时的地形和光学图像采集，且空间分辨率超出衍射极限。这使得能够在一次采集中将表面表征和来自内部细胞组织的信息相结合，从而提供对细胞部分的即时而全面的分析。在这项工作中，将精子固定在聚-L-赖氨酸涂层的盖玻片上，按照标准协议固定，并通过光圈-SNOM在空气中成像。在SNOM图像中，所有精子的特有部分均显示良好的光学特征，与文献数据相比，它们与透射电子显微镜图像中揭示的结构表现出良好的相似性。就健康者而言，异常精子的光学特征明显不同。这项分析揭示了SNOM的潜力，并为将其应用于精子形态变化的高分辨率分析提供了可能，这可能与生殖医学有关。

更新日期：2020-02-13

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