Supplemental Digital Content is available in the text. Although preeclampsia is a common and serious complication of pregnancy, insight into its pathobiology and diagnosis is lacking. Circulating plasma exosomes, which contain RNA and other molecules and have recently become accessible for diagnostics, may be informative in this regard. We tested the hypothesis that preeclampsia may affect the miRNA cargo within circulating maternal blood exosomes. We collected plasma from 60 pregnant women at term, including 20 women with pregnancy complicated by preeclampsia, and 20 women with fetal growth restriction and 20 with healthy pregnancy, serving as controls. We isolated exosomes from the maternal plasma by continuous density gradient ultracentrifugation. Our main outcome variable was exosomal miRNA cargo, analyzed by quantitative polymerase chain reaction-based TaqMan advanced miRNA assay in a card format and the expression of differentially expressed exosomal miRNA in whole plasma from the same participants. We found that 7 miRNA species were differentially expressed in exosomes from women with preeclampsia and those from controls. In contrast, there was no significant difference in exosomal miRNA expression between women with fetal growth restriction and controls. The results were not affected by fetal sex. Only one of the preeclampsia-related, differentially expressed exosomal miRNAs was significantly different in whole plasma miRNA analysis. We concluded that unlike whole plasma miRNA, exosomes extracted from the plasma of women with preeclampsia exhibit a unique miRNA profile, suggesting that plasma exosomal miRNA could provide insight into the pathophysiology of preeclampsia, and may play a role in disease diagnostics.

中文翻译：

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妊娠合并先兆子痫的血浆外泌体中独特的 microRNA 信号


文本中提供了补充数字内容。尽管先兆子痫是一种常见且严重的妊娠并发症，但对其病理学和诊断的深入了解仍然缺乏。循环血浆外泌体含有 RNA 和其他分子，最近已可用于诊断，在这方面可能提供信息。我们测试了先兆子痫可能影响循环母体血液外泌体中 miRNA 含量的假设。我们收集了 60 名足月孕妇的血浆作为对照，其中包括 20 名妊娠合并先兆子痫的妇女、20 名胎儿生长受限的妇女和 20 名健康妊娠的妇女。我们通过连续密度梯度超速离心从母体血浆中分离出外泌体。我们的主要结果变量是外泌体 miRNA 货物，通过基于定量聚合酶链反应的 TaqMan 高级 miRNA 检测以卡的形式进行分析，以及来自同一参与者的全血浆中差异表达的外泌体 miRNA 的表达。我们发现先兆子痫女性和对照女性的外泌体中存在 7 种 miRNA 的差异表达。相比之下，胎儿生长受限的女性和对照组的外泌体 miRNA 表达没有显着差异。结果不受胎儿性别的影响。在全血浆 miRNA 分析中，只有一种与先兆子痫相关、差异表达的外泌体 miRNA 存在显着差异。我们的结论是，与全血浆 miRNA 不同，从先兆子痫女性血浆中提取的外泌体表现出独特的 miRNA 谱，表明血浆外泌体 miRNA 可以深入了解先兆子痫的病理生理学，并可能在疾病诊断中发挥作用。