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Natural sphalerite nanoparticles can accelerate horizontal transfer of plasmid-mediated antibiotic-resistance genes.
Environment International ( IF 10.3 ) Pub Date : 2020-01-27 , DOI: 10.1016/j.envint.2020.105497 Guiying Li 1 , Xiaofang Chen 1 , Hongliang Yin 1 , Wanjun Wang 1 , Po Keung Wong 2 , Taicheng An 1
Environment International ( IF 10.3 ) Pub Date : 2020-01-27 , DOI: 10.1016/j.envint.2020.105497 Guiying Li 1 , Xiaofang Chen 1 , Hongliang Yin 1 , Wanjun Wang 1 , Po Keung Wong 2 , Taicheng An 1
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Minerals and microorganisms are integral parts of natural environments, and they inevitably interact. Antibiotic-resistance genes (ARGs) significantly threaten modern healthcare. However, the effects of natural minerals on ARG propagation in aquatic systems are not fully understood. The present work studied the effects of natural sphalerite (NS) nanoparticles on the horizontal transfer of ARGs from Escherichia coli DH5α (CTX) (donor) to E. coli C600 (Sm) (recipient), and from E. coli DH5α (MCR) (donor) to E. coli C600 (Sm), and their underlying mechanisms. NS particles (0.5-50 mg L-1) induced an NS-concentration-dependent increase in conjugative transfer frequency. The underlying mechanisms associated with the facilitated ARG transfer included the production of intracellular reactive oxygen species, the SOS response, changes in bacterial cell morphology, and alteration of mRNA levels of bacterial cell membrane protein-related genes and genes associated with conjugative ARG transfer. The information herein offers new mechanistic understanding of risks of bacterial resistance resulting from NS.
中文翻译：
天然闪锌矿纳米粒子可以加速质粒介导的抗生素抗性基因的水平转移。
矿物质和微生物是自然环境的组成部分，它们不可避免地相互作用。抗生素抗性基因（ARG）严重威胁着现代医疗保健。但是，尚未完全了解天然矿物对水生系统中ARG繁殖的影响。本工作研究了天然闪锌矿（NS）纳米颗粒对ARGs从大肠杆菌DH5α（CTX）（供体）到大肠杆菌C600（Sm）（受体）和大肠杆菌DH5α（MCR）的水平转移的影响（供体）大肠杆菌C600（Sm）及其潜在机制。NS颗粒（0.5-50 mg L-1）引起NS浓度依赖性的共轭转移频率增加。与促进ARG转移相关的潜在机制包括细胞内活性氧的产生，SOS反应，细菌细胞形态的变化，以及细菌细胞膜蛋白相关基因和与共轭ARG转移相关的基因的mRNA水平的变化。本文中的信息为由NS引起的细菌耐药性风险提供了新的机理理解。
更新日期：2020-01-27
中文翻译：
天然闪锌矿纳米粒子可以加速质粒介导的抗生素抗性基因的水平转移。
矿物质和微生物是自然环境的组成部分，它们不可避免地相互作用。抗生素抗性基因（ARG）严重威胁着现代医疗保健。但是，尚未完全了解天然矿物对水生系统中ARG繁殖的影响。本工作研究了天然闪锌矿（NS）纳米颗粒对ARGs从大肠杆菌DH5α（CTX）（供体）到大肠杆菌C600（Sm）（受体）和大肠杆菌DH5α（MCR）的水平转移的影响（供体）大肠杆菌C600（Sm）及其潜在机制。NS颗粒（0.5-50 mg L-1）引起NS浓度依赖性的共轭转移频率增加。与促进ARG转移相关的潜在机制包括细胞内活性氧的产生，SOS反应，细菌细胞形态的变化，以及细菌细胞膜蛋白相关基因和与共轭ARG转移相关的基因的mRNA水平的变化。本文中的信息为由NS引起的细菌耐药性风险提供了新的机理理解。
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