There has been a considerable development in microfluidic based immunodiagnostics over the past few years which has greatly favored the growth of novel point-of-care-testing (POCT). However, the realization of an inexpensive, low-power POCT needs cheap and disposable microfluidic devices that can perform autonomously with minimum user intervention. This work, for the first time, reports the development of a new microchannel capillary flow assay (MCFA) platform that can perform chemiluminescence based ELISA with lyophilized chemiluminescent reagents. This new MCFA platform exploits the ultra-high sensitivity of chemiluminescent detection while eliminating the shortcomings associated with liquid reagent handling, control of assay sequence and user intervention. The functionally designed microchannels along with adequate hydrophilicity produce a sequential flow of assay reagents and autonomously performs the ultra-high sensitive chemiluminescence based ELISA for the detection of malaria biomarker such as PfHRP2. The MCFA platform with no external flow control and simple chemiluminescence detection can easily communicate with smartphone via USB-OTG port using a custom-designed optical detector. The use of the smartphone for display, data transfer, storage and analysis, as well as the source of power allows the development of a smartphone based POCT analyzer for disease diagnostics. This paper reports a limit of detection (LOD) of 8 ng/mL by the smartphone analyzer which is sensitive enough to detect active malarial infection. The MCFA platform developed with the smartphone analyzer can be easily customized for different biomarkers, so a hand-held POCT for various infectious diseases can be envisaged with full networking capability at low cost.

在过去几年中，基于微流体的免疫诊断学取得了长足的发展，极大地促进了新型即时检测 (POCT) 的发展。然而，实现廉价、低功耗的 POCT 需要廉价且一次性的微流体设备，这些设备可以在用户干预最少的情况下自主执行。这项工作首次报告了一种新的微通道毛细管流动测定 (MCFA) 平台的开发，该平台可以使用冻干化学发光试剂进行基于化学发光的 ELISA。这种新的 MCFA 平台利用了化学发光检测的超高灵敏度，同时消除了与液体试剂处理、测定序列控制和用户干预相关的缺点。功能设计的微通道以及足够的亲水性产生连续的测定试剂流，并自主执行基于超高灵敏度化学发光的 ELISA，用于检测疟疾生物标志物，如 PfHRP2。没有外部流量控制和简单化学发光检测的 MCFA 平台可以使用定制设计的光学检测器通过 USB-OTG 端口与智能手机轻松通信。使用智能手机进行显示、数据传输、存储和分析以及电源允许开发基于智能手机的用于疾病诊断的 POCT 分析仪。本文报告了智能手机分析仪的检测限 (LOD) 为 8 ng/mL，其灵敏度足以检测活动性疟疾感染。