Three new C₂₀-diterpenoid alkaloids with a sulfonic acid unit, named aconicarmisulfonines B and C (1 and 2) and chuanfusulfonine A (3), respectively, were isolated from the Aconitum carmichaelii lateral roots (“fu zi” in Chinese). Structures of 1–3 were determined by spectroscopic data analysis. Intriguing chemical properties and reactions were observed for the C₂₀-diterpenoid alkaloids: (a) specific selective nucleophilic addition of the carbonyl (C-12) in 1 with CD₃OD; (b) interconversion between 1 and 2 in D₂O; (c) stereo- and/or regioselective deuterations of H-11α in 1–3 and both H-11α and H-11β in aconicarmisulfonine A (4); (d) TMSP-2,2,3,3-d₄ promoted cleavage of the C-12−C-13 bond of 4 in D₂O; (e) dehydrogenation of 4 in pyridine-d₅, and (f) Na₂SO₃-assisted dehydrogenation and N-deethylation of songorine (5, a putative precursor of 1–4). Biogenetically, 1 and 2 are correlated with 4, for which the same novel carbon skeleton is proposed to be derived from semipinacol rearrangements via migrations of C-13−C-16 and C-15−C-16 bonds of the napelline-type skeleton, respectively. Meanwhile, 3 is a highly possible precursor or a concurrent product in the biosynthetic pathways of 1, 2, and 4. In the acetic acid-induced mice writhing assay, at 1.0 mg/kg (i.p.), compounds 1, 2, 5, 5a, and 5b exhibited analgesic effects against mice writhing.

从乌头侧根中分离得到 3 个新的带有磺酸单元的 C ₂₀ -二萜生物碱，分别命名为乌头磺胺 B 和 C ( 1和2 ) 以及川附磺宁 A ( 3 )。通过光谱数据分析确定1-3的结构。观察到 C ₂₀ -二萜生物碱的有趣化学性质和反应：(a) 1中的羰基 (C-12) 与 CD ₃ OD 发生特异性选择性亲核加成； (b)D ₂ O中1和2之间的相互转化； (c) 1-3中 H-11 α以及乌头磺胺 A 中 H-11 α和 H-11 β的立体和/或区域选择性氘化 ( 4 )； (d) TMSP-2,2,3,3- d ₄促进 D ₂ O 中4的 C-12−C-13 键裂解； (e) 4在吡啶-d ₅中脱氢，以及(f) Na ₂ SO ₃辅助松戈林脱氢和N-脱乙基化（ 5 ，假定的1 – 4前体）。 在生物遗传学上， 1和2与4相关，其中相同的新型碳骨架被认为是通过napelline型骨架的C-13−C-16和C-15−C-16键的迁移从半频那醇重排衍生而来， 分别。同时， 3很可能是1、2和4生物合成途径中的前体或同时产物。在乙酸诱导的小鼠扭体测定中，在1.0mg /kg(ip )下，化合物1、2、5、5a和5b对小鼠扭体表现出镇痛作用。