Fluorescent microspheres (FMs) are widely employed in diagnostics and life sciences research; here, we investigated the effect of capillary coating, polymer concentration, electric field strength, and sample concentration on the separation performance of 1.0 μm FMs in hydroxyethyl cellulose (HEC) by capillary electrophoresis (CE). Results showed that (1) capillary coating could enhance the fluorescence signal. (2) For HEC with the same molecular weight, the higher HEC concentration is, the later the first peak appears in the electropherogram. (3) When FMs are diluted, increasing the electric field strength can enhance the migration speed and reduce the aggregation of FMs. (4) The number of FMs calculated is close to the theoretical value when it is diluted 10,000 times. The optimum conditions for CE were as follows: 6 cm/8 cm of effective length and total length of the coated capillary, 0.3% HEC (1300 k), and 300 V/cm of electric field strength. Such a study is helpful for the development of a FM counting system. Graphical abstract.

中文翻译：

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通过毛细管电泳分离亚细胞荧光微球。

荧光微球（FMs）被广泛用于诊断和生命科学研究。在这里，我们研究了毛细管电泳（CE）对毛细管涂层，聚合物浓度，电场强度和样品浓度对1.0μmFMs在羟乙基纤维素（HEC）中分离性能的影响。结果表明：（1）毛细管涂层可以增强荧光信号。（2）对于相同分子量的HEC，HEC浓度越高，在电泳图中第一个峰出现的时间越晚。（3）当稀释FM时，增加电场强度可以提高迁移速度并减少FM的聚集。（4）10,000倍稀释后的FM数接近理论值。CE的最佳条件如下：有效长度和涂层毛细管的总长度为6 cm / 8 cm，0.3％HEC（1300 k），电场强度为300 V / cm。这样的研究对于FM计数系统的发展是有帮助的。图形概要。