Sucrose utilization has been established in Escherichia coli strains by expression of Mannheimia succiniciproducens β-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coli strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] from sucrose. When recombinant E. coli strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coli strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P(3HB-co-LA) from sucrose. Among the examined E. coli strains, recombinant E. coli XL1-Blue produced the highest contents of P(3HB) (53.60 ± 2.55 wt%) and P(3HB-co-LA) (29.44 ± 0.39 wt%). In the batch fermentations, recombinant E. coli XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt% P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a cost-efficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source.

通过表达琥珀酸曼尼海姆菌产生的β-果糖呋喃糖苷酶（SacC），已在大肠杆菌菌株中建立了蔗糖利用，该酶将蔗糖水解成葡萄糖和果糖。重组大肠杆菌（E.coli）检查它们的能力，以产生聚（3-羟基丁酸酯）[P（3HB）]和聚（3-羟基丁酸酯，可以利用蔗糖菌株共-乳酸）[P（3HB-共-LA）]从蔗糖。当将重组大肠杆菌表达菌株富养产碱PhaCAB和SACC在含有20g / L的蔗糖MR培养基中培养，所有的重组大肠杆菌菌株能够从蔗糖生产P（3HB）。另外，重组大肠杆菌表达假单胞菌sp。的菌株 MBEL 6-19 PhaC1437，丙酸梭状芽胞杆菌Pct540，富氧罗汉果菌PhaAB酶与SacC一起可以从蔗糖中产生P（3HB - co -LA）。在所检查的大肠杆菌菌株中，重组大肠杆菌XL1-Blue产生最高含量的P（3HB）（53.60±2.55 wt％）和P（3HB - co -LA）（29.44±0.39 wt％）。在分批发酵中，重组大肠杆菌XL1-Blue菌株完全消耗了20 g / L的蔗糖作为唯一碳源，并支持生产3.76 g / L的P（3HB）和1.82 g / L的P（3HB-共-LA）与38.21重量％的P（3HB）和20.88重量％的P（3HB-共-LA）内容。在这项研究中开发的重组大肠杆菌菌株可用于建立具有成本效益的生物精炼厂，以从蔗糖生产聚羟基链烷酸酯（PHA），蔗糖是一种丰富而廉价的碳源。