Extracellular matrix (ECM) has a major role in the structural support and cellular processes of organs and tissues. Proteins extracted from the ECM have been used to fabricate different scaffolds for tissue engineering applications. The aims of the present study were to extract, characterize and fabricate a new class of hydrogel with proteins isolated from pig bone ECM and combine them with a synthetic polymer so it could be used to promote bone regeneration. Porcine bone demineralized and digested extracellular matrix (pddECM) containing collagen type I was produced, optimized and sterilized with high pressurized CO2 method. The pddECM was further blended with 20% w/v polyethylene glycol diacrylate (PEGDA) to create an injectable semi interpenetrating polymer network (SIPN) scaffold with enhanced physicochemical properties. The blend tackled the shortfall of natural polymers, such as lack of structural stability and fast degradation, preserving its structure in more than 90% after 30 days of incubation; thus, increasing the material endurance in a simulated physiological environment. The manufactured injectable hydrogel showed high cytocompatibility with hOb and SaOs-2 cells, promoting osteogenic proliferation within 21 days of culture. The hydrogel had a high compression modulus of 520 kPa, low swelling (5.3 mg/mg) and millimetric volume expansion (19.5%), all of which are favorable characteristics for bone regeneration applications.

中文翻译：

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可注射的猪骨去矿物质和消化的细胞外基质-PEGDA水凝胶共混物，用于骨骼再生。

细胞外基质（ECM）在器官和组织的结构支持和细胞过程中起主要作用。从ECM提取的蛋白质已被用于制造用于组织工程应用的不同支架。本研究的目的是使用从猪骨ECM中分离出的蛋白质提取，表征和制造一类新型水凝胶，并将其与合成聚合物结合，从而可用于促进骨再生。用高压二氧化碳法生产，优化和灭菌了含有I型胶原的猪骨去矿物质和消化的细胞外基质（pddECM）。将pddECM与20％w / v聚乙二醇二丙烯酸酯（PEGDA）进一步混合，以创建具有增强的理化性质的可注射半互穿聚合物网络（SIPN）支架。共混物解决了天然聚合物不足的问题，例如缺乏结构稳定性和快速降解，在孵育30天后可将其结构保持90％以上；因此，在模拟的生理环境中增加了材料的耐力。制成的可注射水凝胶显示出与hOb和SaOs-2细胞的高度细胞相容性，并在培养的21天之内促进了成骨细胞的增殖。该水凝胶具有520 kPa的高压缩模量，低溶胀（5.3 mg / mg）和毫米级体积膨胀（19.5％），所有这些都是骨骼再生应用的有利特性。在模拟的生理环境中提高材料的耐力。制成的可注射水凝胶显示出与hOb和SaOs-2细胞的高度细胞相容性，并在培养的21天之内促进了成骨细胞的增殖。该水凝胶具有520 kPa的高压缩模量，低溶胀（5.3 mg / mg）和毫米级体积膨胀（19.5％），所有这些都是骨骼再生应用的有利特性。在模拟的生理环境中提高材料的耐力。制成的可注射水凝胶显示出与hOb和SaOs-2细胞的高度细胞相容性，并在培养的21天之内促进了成骨细胞的增殖。该水凝胶具有520 kPa的高压缩模量，低溶胀（5.3 mg / mg）和毫米级体积膨胀（19.5％），所有这些都是骨骼再生应用的有利特性。