当前位置: X-MOL 学术Fish Shellfish Immun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Identification, expression profiling and functional characterization of interleukin 11a ortholog from redlip mullet Liza haematocheila: Insight into its roles in the inflammation and apoptosis regulation.
Fish & Shellfish Immunology ( IF 4.1 ) Pub Date : 2020-01-27 , DOI: 10.1016/j.fsi.2020.01.045
T D W Kasthuriarachchi 1 , Qiang Wan 1 , Jehee Lee 1
Affiliation  

Interleukin 11 (IL-11) is a secretory cytokine with pleotropic properties, including anti-inflammatory and anti-apoptotic functions. This study aimed to functionally characterize a teleostean IL-11a ortholog from redlip mullet (LhIL-11a) through bioinformatic analysis, transcriptional expression profiling and protein function assays. The deduced LhIL-11a protein sequence is 200 amino acids long, with a predicted molecular weight of 23.168 kDa. Multiple sequence alignment indicates that LhIL-11a has a typical four-bundle architecture of α-helixes as observed in other IL-11s. The identity-similarity matrix show a higher identity between LhIL-11a and other fish IL-11a sequences. Phylogenetic analysis demonstrated that LhIL-11a falls within a clade including other fish counterparts. In the tissue distribution analysis, the highest constitutive expression of LhIL-11a mRNA was observed in the mullet gastrointestinal tract and brain tissues. Following the challenges with LPS, poly I:C and Lactococcus garvie, the transcription levels of LhIL-11a were significantly upregulated in both PBCs and liver. In the biological functional assay, recombinant LhIL-11a protein showed strong activities of suppressing pro-inflammatory cytokines and apoptotic gene expression in mullet kidney cells and reducing LPS stimulated NO production in murine macrophage cells. Overall, the findings in this study provide the experimental clues to understanding the functional roles of fish IL-11a in inflammation and apoptosis regulation during host defense against invading microbial pathogens.

中文翻译:

红唇鱼Liza haematocheila的白介素11a直系同源物的鉴定,表达谱和功能表征:洞察其在炎症和凋亡调控中的作用。

白介素11(IL-11)是一种具有多效性的分泌型细胞因子,包括抗炎和抗凋亡功能。这项研究旨在通过生物信息学分析,转录表达谱和蛋白质功能分析从红唇鱼(LhIL-11a)功能上鉴定硬骨鱼IL-11a直系同源物。推导的LhIL-11a蛋白序列长200个氨基酸,预测分子量为23.168 kDa。多个序列比对表明,如在其他IL-11中所观察到的,LhIL-11a具有典型的四束α-螺旋结构。同一性相似度矩阵显示LhIL-11a与其他鱼类IL-11a序列之间具有较高的同一性。系统发育分析表明,LhIL-11a属于进化枝,包括其他鱼类。在组织分布分析中 在h鱼胃肠道和脑组织中观察到最高的LhIL-11a mRNA组成型表达。在LPS,poly I:C和garactococcus garvie挑战之后,Phs和肝脏中LhIL-11a的转录水平均显着上调。在生物学功能测定中,重组LhIL-11a蛋白显示出强大的抑制activities鱼肾细胞促炎性细胞因子和凋亡基因表达并减少LPS刺激鼠巨噬细胞NO生成的活性。总的来说,这项研究的发现为理解鱼IL-11a在宿主防御入侵的微生物病原体过程中在炎症和凋亡调控中的功能性作用提供了实验线索。在LPS,poly I:C和garactococcus garvie挑战之后,Phs和肝脏中LhIL-11a的转录水平均显着上调。在生物学功能测定中,重组LhIL-11a蛋白显示出强大的抑制activities鱼肾细胞促炎性细胞因子和凋亡基因表达并减少LPS刺激鼠巨噬细胞NO生成的活性。总的来说,这项研究的发现为理解鱼IL-11a在宿主防御入侵的微生物病原体过程中在炎症和凋亡调控中的功能性作用提供了实验线索。在LPS,poly I:C和garactococcus garvie挑战之后,Phs和肝脏中LhIL-11a的转录水平均显着上调。在生物学功能测定中,重组LhIL-11a蛋白显示出强大的抑制activities鱼肾细胞促炎性细胞因子和凋亡基因表达并减少LPS刺激鼠巨噬细胞NO生成的活性。总的来说,这项研究的发现为理解鱼IL-11a在宿主防御入侵的微生物病原体过程中在炎症和凋亡调控中的功能性作用提供了实验线索。重组LhIL-11a蛋白显示出强大的抑制of鱼肾细胞促炎性细胞因子和凋亡基因表达并减少LPS刺激的鼠巨噬细胞NO生成的活性。总的来说,这项研究的发现为理解鱼IL-11a在宿主防御入侵的微生物病原体过程中在炎症和凋亡调控中的功能性作用提供了实验线索。重组LhIL-11a蛋白显示出强大的抑制of鱼肾细胞促炎性细胞因子和凋亡基因表达并减少LPS刺激的鼠巨噬细胞NO生成的活性。总的来说,这项研究的发现为理解鱼IL-11a在宿主防御入侵的微生物病原体过程中在炎症和凋亡调控中的功能性作用提供了实验线索。
更新日期:2020-01-27
down
wechat
bug