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Genomic and functional insights into the adaptation and survival of Chryseobacterium sp. strain PMSZPI in uranium enriched environment.
Ecotoxicology and Environmental Safety ( IF 6.2 ) Pub Date : 2020-01-27 , DOI: 10.1016/j.ecoenv.2020.110217 Devanshi Khare 1 , Rakshak Kumar 2 , Celin Acharya 1
Ecotoxicology and Environmental Safety ( IF 6.2 ) Pub Date : 2020-01-27 , DOI: 10.1016/j.ecoenv.2020.110217 Devanshi Khare 1 , Rakshak Kumar 2 , Celin Acharya 1
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Metal enriched areas represent important and dynamic microbiological ecosystems. In this study, the draft genome of a uranium (U) tolerant bacterium, Chryseobacterium sp. strain PMSZPI, isolated from the subsurface soil of Domiasiat uranium ore deposit in Northeast India, was analyzed. The strain revealed a genome size of 3.8 Mb comprising of 3346 predicted protein-coding genes. The analysis indicated high abundance of genes associated with metal resistance and efflux, transporters, phosphatases, antibiotic resistance, polysaccharide synthesis, motility, protein secretion systems, oxidoreductases and DNA repair. Comparative genomics with other closely related Chryseobacterium strains led to the identification of unique inventory of genes which were of adaptive significance in PMSZPI. Consistent with the genome analysis, PMSZPI showed superior tolerance to uranium and other heavy metals. The metal exposed cells exhibited transcriptional induction of metal translocating PIB ATPases suggestive of their involvement in metal resistance. Efficient U binding (~90% of 100 μM U) and U bioprecipitation (~93-94% of 1 mM U at pH 5, 7 and 9) could be attributed as uranium tolerance strategies in PMSZPI. The strain demonstrated resistance to a large number of antibiotics which was in agreement with in silico prediction. Reduced gliding motility in the presence of cadmium and uranium, enhanced biofilm formation on uranium exposure and tolerance to 1.5 kGy of 60Co gamma radiation were perceived as adaptive responses in PMSZPI. Overall, the positive correlation observed between uranium/metal tolerance abilities predicted using genome analysis and the functional characterization reinforced the multifaceted adaptation strategies employed by PMSZPI for its survival in the soil of uranium ore deposit comprising of high concentrations of uranium and other heavy metals.
中文翻译：
基因和功能的见解，以适应水晶细菌的生存。富铀环境中的PMSZPI菌株。
金属富集区代表着重要的动态微生物生态系统。在这项研究中，铀（U）耐性细菌Chryseobacterium sp。的基因组草图。从印度东北的多米亚西特铀矿床地下土壤中分离出的PMSZPI菌株进行了分析。该菌株的基因组大小为3.8 Mb，包含3346个预测的蛋白质编码基因。分析表明与金属抗性和外排，转运蛋白，磷酸酶，抗生素抗性，多糖合成，运动性，蛋白质分泌系统，氧化还原酶和DNA修复相关的基因高度丰富。与其他紧密相关的金黄色葡萄球菌菌株的比较基因组学导致鉴定出独特的基因库存，这些基因在PMSZPI中具有适应性意义。与基因组分析一致，PMSZPI显示出对铀和其他重金属的优异耐受性。暴露于金属的细胞表现出金属易位PIB ATPase的转录诱导，表明它们参与了金属抗性。有效的U结合（〜100μMU的〜90％）和U生物沉淀（在pH 5、7和9下1 mM U的〜93-94％）可以归因于PMSZPI中的铀耐受策略。该菌株显示出对多种抗生素的抗性，这与计算机模拟预测相符。在PMSZPI中，镉和铀存在下滑行运动性降低，铀暴露下生物膜形成增强以及对1.5 kGy的60Coγ射线耐受性被认为是适应性反应。总体，
更新日期：2020-01-27
中文翻译：
基因和功能的见解，以适应水晶细菌的生存。富铀环境中的PMSZPI菌株。
金属富集区代表着重要的动态微生物生态系统。在这项研究中，铀（U）耐性细菌Chryseobacterium sp。的基因组草图。从印度东北的多米亚西特铀矿床地下土壤中分离出的PMSZPI菌株进行了分析。该菌株的基因组大小为3.8 Mb，包含3346个预测的蛋白质编码基因。分析表明与金属抗性和外排，转运蛋白，磷酸酶，抗生素抗性，多糖合成，运动性，蛋白质分泌系统，氧化还原酶和DNA修复相关的基因高度丰富。与其他紧密相关的金黄色葡萄球菌菌株的比较基因组学导致鉴定出独特的基因库存，这些基因在PMSZPI中具有适应性意义。与基因组分析一致，PMSZPI显示出对铀和其他重金属的优异耐受性。暴露于金属的细胞表现出金属易位PIB ATPase的转录诱导，表明它们参与了金属抗性。有效的U结合（〜100μMU的〜90％）和U生物沉淀（在pH 5、7和9下1 mM U的〜93-94％）可以归因于PMSZPI中的铀耐受策略。该菌株显示出对多种抗生素的抗性，这与计算机模拟预测相符。在PMSZPI中，镉和铀存在下滑行运动性降低，铀暴露下生物膜形成增强以及对1.5 kGy的60Coγ射线耐受性被认为是适应性反应。总体，
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