Rapid fabrication of precise high-throughput filters from membrane protein nanosheets,Nature Materials

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Rapid fabrication of precise high-throughput filters from membrane protein nanosheets
Nature Materials ( IF 37.2 ) Pub Date : 2020-01-27 , DOI: 10.1038/s41563-019-0577-z
Yu-Ming Tu _{1,

2} , Woochul Song _{1,

2} , Tingwei Ren ₁ , Yue-Xiao Shen ₃ , Ratul Chowdhury ₁ , Prasangi Rajapaksha ₄ , Tyler E Culp ₁ , Laxmicharan Samineni _{1,

2} , Chao Lang _{1,

5} , Alina Thokkadam ₆ , Drew Carson ₁ , Yuxuan Dai ₁ , Arwa Mukthar ₇ , Miaoci Zhang ₁ , Andrey Parshin ₈ , Janna N Sloand ₉ , Scott H Medina ₉ , Mariusz Grzelakowski ₈ , Dibakar Bhattacharya ₁₀ , William A Phillip ₁₁ , Enrique D Gomez ₁ , Robert J Hickey _{5,

12} , Yinai Wei ₄ , Manish Kumar _{1,

12,

13,

14}

Affiliation

Department of Chemical Engineering, Pennsylvania State University, University Park, PA, USA.
Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
Department of Civil, Environmental, & Construction Engineering, Texas Tech University, Lubbock, TX, USA.
Department of Chemistry, University of Kentucky, Lexington, KY, USA.
Department of Materials Science and Engineering, The Pennsylvania State University, University Park, PA, USA.
Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, NJ, USA.
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA.
Applied Biomimetic, Gaithersburg, MD, USA.
Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA.
Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY, USA.
Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, USA.
Materials Research Institute, Pennsylvania State University, University Park, PA, USA.
Department of Civil and Environmental Engineering, Pennsylvania State University, University Park, PA, USA.
Department of Civil, Architectural and Environmental Engineering, The University of Texas at Austin, Austin, TX, USA.

Biological membranes are ideal for separations as they provide high permeability while maintaining high solute selectivity due to the presence of specialized membrane protein (MP) channels. However, successful integration of MPs into manufactured membranes has remained a significant challenge. Here, we demonstrate a two-hour organic solvent method to develop 2D crystals and nanosheets of highly packed pore-forming MPs in block copolymers (BCPs). We then integrate these hybrid materials into scalable MP-BCP biomimetic membranes. These MP-BCP nanosheet membranes maintain the molecular selectivity of the three types of β-barrel MP channels used, with pore sizes of 0.8 nm, 1.3 nm, and 1.5 nm. These biomimetic membranes demonstrate water permeability that is 20–1,000 times greater than that of commercial membranes and 1.5–45 times greater than that of the latest research membranes with comparable molecular exclusion ratings. This approach could provide high performance alternatives in the challenging sub-nanometre to few-nanometre size range.

中文翻译：

从膜蛋白纳米片快速制造精确的高通量过滤器

生物膜是分离的理想选择，因为它们提供高渗透性，同时由于存在专门的膜蛋白 (MP) 通道而保持高溶质选择性。然而，将 MPs 成功整合到制造的膜中仍然是一项重大挑战。在这里，我们展示了一种两小时的有机溶剂方法来开发嵌段共聚物 (BCP) 中高度堆积的成孔 MPs 的二维晶体和纳米片。然后，我们将这些混合材料集成到可扩展的 MP-BCP 仿生膜中。这些 MP-BCP 纳米片膜保持了所使用的三种 β-桶 MP 通道的分子选择性，孔径分别为 0.8 nm、1.3 nm 和 1.5 nm。这些仿生膜的透水性比商业膜高 20-1,000 倍，1。比具有可比分子排阻等级的最新研究膜高 5-45 倍。这种方法可以在具有挑战性的亚纳米到几纳米尺寸范围内提供高性能替代方案。

更新日期：2020-01-27

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