FERARI is required for Rab11-dependent endocytic recycling.,Nature Cell Biology

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FERARI is required for Rab11-dependent endocytic recycling.
Nature Cell Biology ( IF 17.3 ) Pub Date : 2020-01-27 , DOI: 10.1038/s41556-019-0456-5
Jachen A Solinger ₁ , Harun-Or Rashid ₁ , Cristina Prescianotto-Baschong ₁ , Anne Spang ₁

Affiliation

Endosomal transport is essential for cellular organization and compartmentalization and cell-cell communication. Sorting endosomes provide a crossroads for various trafficking pathways and determine recycling, secretion or degradation of proteins. The organization of these processes requires membrane-tethering factors to coordinate Rab GTPase function with membrane fusion. Here, we report a conserved tethering platform that acts in the Rab11 recycling pathways at sorting endosomes, which we name factors for endosome recycling and Rab interactions (FERARI). The Rab-binding module of FERARI consists of Rab11FIP5 and rabenosyn-5/RABS-5, while the SNARE-interacting module comprises VPS45 and VIPAS39. Unexpectedly, the membrane fission protein EHD1 is also a FERARI component. Thus, FERARI appears to combine fusion activity through the SM protein VPS45 with pinching activity through EHD1 on SNX-1-positive endosomal membranes. We propose that coordination of fusion and pinching through a kiss-and-run mechanism drives cargo at endosomes into recycling pathways.

中文翻译：

FERARI是Rab11依赖性内吞再循环所必需的。

内体转运对于细胞的组织和分隔以及细胞间的通信至关重要。内体的分类为各种运输途径提供了十字路口，并确定了蛋白质的再循环，分泌或降解。这些过程的组织需要膜束缚因子来协调Rab GTPase功能与膜融合。在这里，我们报告了一个保守的拴系平台，该平台在Rab11回收途径中对内体进行分选，我们将其命名为内体回收和Rab相互作用的因素（FERARI）。FERARI的Rab绑定模块由Rab11FIP5和rabenosyn-5 / RABS-5组成，而SNARE交互模块包括VPS45和VIPAS39。出乎意料的是，膜裂变蛋白EHD1也是FERARI的组成部分。从而，FERARI似乎将SM蛋白VPS45的融合活性与ENX1对SNX-1阳性内体膜的捏合活性结合在一起。我们建议融合和捏合的吻和运行机制的协调驱动体内的货物进入回收途径。

更新日期：2020-01-27

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