1H magnetic resonance spectroscopy of 2H-to-1H exchange quantifies the dynamics of cellular metabolism in vivo.,Nature Biomedical Engineering

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1H magnetic resonance spectroscopy of 2H-to-1H exchange quantifies the dynamics of cellular metabolism in vivo.
Nature Biomedical Engineering ( IF 26.8 ) Pub Date : 2020-01-27 , DOI: 10.1038/s41551-019-0499-8
Laurie J Rich ₁ , Puneet Bagga ₁ , Neil E Wilson ₁ , Mitchell D Schnall ₁ , John A Detre _{1,

2} , Mohammad Haris _{1,

3,

4} , Ravinder Reddy ₁

Affiliation

Quantitative mapping of the in vivo dynamics of cellular metabolism via non-invasive imaging contributes to our understanding of the initiation and progression of diseases associated with dysregulated metabolic processes. Current methods for imaging cellular metabolism are limited by low sensitivities, costs or the use of specialized hardware. Here, we introduce a method that captures the turnover of cellular metabolites by quantifying signal reductions in proton magnetic resonance spectroscopy (MRS) resulting from the replacement of 1H with 2H. The method, which we termed quantitative exchanged-label turnover MRS, only requires deuterium-labelled glucose and standard magnetic resonance imaging scanners, and with a single acquisition provides steady-state information and metabolic rates for several metabolites. We used the method to monitor glutamate, glutamine, γ-aminobutyric acid and lactate in the brains of unaffected and glioma-bearing rats following the administration of 2H2-labelled glucose and 2H3-labelled acetate. Quantitative exchanged-label turnover MRS should broaden the applications of routine 1H MRS.

中文翻译：

2H到1H交换的1H磁共振波谱可量化体内细胞代谢的动力学。

通过无创成像定量分析细胞代谢的体内动力学，有助于我们理解与代谢过程失调有关的疾病的发生和发展。低灵敏度，低成本或使用专用硬件限制了目前用于细胞代谢成像的方法。在这里，我们介绍一种通过量化由1H替换为2H导致的质子磁共振波谱（MRS）信号减少来捕获细胞代谢物更新的方法。我们称其为定量交换标签转换MRS的方法，仅需要氘标记的葡萄糖和标准的磁共振成像扫描仪，一次采集即可提供几种代谢物的稳态信息和代谢率。我们使用该方法监测了未患病和患有神经胶质瘤的大鼠大脑中的谷氨酸，谷氨酰胺，γ-氨基丁酸和乳酸，这些药物是在施用2H2标记的葡萄糖和2H3标记的乙酸盐后给药的。定量交换标签更换MRS应该扩大常规1H MRS的应用。

更新日期：2020-01-27

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