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Preparation of Aptamer Responsive DNA Functionalized Hydrogels for the Sensitive Detection of α-Fetoprotein Using SERS Method.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2020-01-31 , DOI: 10.1021/acs.bioconjchem.9b00874
Qi Wang 1 , Yongjun Hu 1 , Ningjing Jiang 1 , Junjie Wang 1 , Meng Yu 1 , Xiumei Zhuang 1
Affiliation  

Hepatocellular carcinoma (HCC), which is one of the three major cancers, has attracted growing attention due to its high mortality, health care cost, and circumscribed therapeutic methods. Hence, the development of a fast, accurate, and flexible method to detect α-fetoprotein (AFP), the specific marker of HCC, is significant for diagnosis and treatment of cancer. Here, we constructed a novel SERS biosensing platform combining the target-responsive DNA hydrogel for the sensitive detection of AFP. The linker strand in DNA hydrogel is an aptamer that can specifically recognize AFP and accurately control the release of immunoglobulin G (IgG) encapsulated in hydrogel. In the presence of AFP, the hydrogels were disentangled and the IgG was released. Thereafter, the released IgG was captured by SERS probes and biofunctional magnetic beads through formation of sandwich-like structures, resulting in the signal of Raman tags decreasing in the supernatant after magnetic separation. Due to the ultrahigh sensitivity of the SERS biosensor, the proposed method has a wide detection linear range (50 pg/mL to 0.5 μg/mL) and a detection limit down to 50 pg/mL. Moreover, the sequence of the linker strand in the DNA hydrogel can be specifically encoded into a new aptamer that responds to other cancer markers. This convenient and inexpensive detection method provides a new strategy for the detection of tumor markers.

中文翻译:

利用SERS方法制备用于敏感检测α-甲胎蛋白的适体响应性DNA功能化水凝胶。

肝细胞癌(HCC)是三大主要癌症之一,由于其高死亡率,医疗保健费用和限制的治疗方法,引起了越来越多的关注。因此,开发一种快速,准确和灵活的方法来检测HCC的特异性标志物-甲胎蛋白(AFP),对于癌症的诊断和治疗具有重要意义。在这里,我们构建了一个新型的SERS生物传感平台,结合了靶反应性DNA水凝胶,用于AFP的灵敏检测。DNA水凝胶中的接头链是一种适体,它可以特异性识别AFP并精确控制封装在水凝胶中的免疫球蛋白G(IgG)的释放。在AFP存在下,将水凝胶解开并释放IgG。之后,SERS探针和生物功能性磁珠通过形成夹心状结构捕获释放的IgG,导致磁分离后上清液中拉曼标签的信号减少。由于SERS生物传感器的超高灵敏度,所提出的方法具有宽的检测线性范围(50 pg / mL至0.5μg/ mL)和低至50 pg / mL的检测限。而且,DNA水凝胶中的接头链的序列可以被特异性地编码成对其他癌症标志物有反应的新适体。这种方便且廉价的检测方法为检测肿瘤标志物提供了新的策略。所提出的方法具有宽的检测线性范围(50 pg / mL至0.5μg/ mL),检测限低至50 pg / mL。而且,DNA水凝胶中的接头链的序列可以被特异性地编码成对其他癌症标志物有反应的新适体。这种方便且廉价的检测方法为检测肿瘤标志物提供了新的策略。所提出的方法具有宽的检测线性范围(50 pg / mL至0.5μg/ mL),检测限低至50 pg / mL。而且,DNA水凝胶中的接头链的序列可以被特异性地编码成对其他癌症标志物有反应的新适体。这种方便且廉价的检测方法为检测肿瘤标志物提供了新的策略。
更新日期:2020-02-03
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