In presented study, various chitosan derivatives containing covalently bounded gallic acid were obtained: chitosan with gallic acid (CG), quaternized chitosan with gallic acid (QCG), and succinylated chitosan with gallic acid (SCG). Chitosan derivatives were used as stabilizing and reducing agents in the synthesis of silver nanoparticles (AgNPs). The dimensional characteristics of nanomaterials were determined by transmission electron (TEM), dynamic light scattering (DLS) and atomic force (AFM) microscopy, antibacterial activity (against E. coli, S. epidermidis), cytotoxicity (HaCaT, Colo 357 cell lines) and hemocompatibility. Among all samples, QCG-AgNPs showed low toxicity in the range of studied concentrations (3.125-100 μg/ml) high stability of nanoparticle for 4 months (according to UV.spectroscopy data) the highest antibacterial activity against S. epidermidis (3.91 μg/ml). The high antibacterial activity, stability, and simplicity of the process of producing AgNPs based on the QCG derivative reveals that a new method for producing modified AgNPs deserves future consideration.

中文翻译：

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使用没食子酸共轭壳聚糖衍生物合成银纳米粒子。

在目前的研究中，获得了各种含有共价结合的没食子酸的壳聚糖衍生物：带有没食子酸的壳聚糖（CG），带有没食子酸的季铵化壳聚糖（QCG）和带有没食子酸的琥珀酰化壳聚糖（SCG）。壳聚糖衍生物在银纳米颗粒（AgNPs）的合成中用作稳定剂和还原剂。纳米材料的尺寸特性由透射电子（TEM），动态光散射（DLS）和原子力（AFM）显微镜，抗菌活性（针对大肠杆菌，表皮葡萄球菌），细胞毒性（HaCaT，Colo 357细胞系）确定。和血液相容性。在所有样品中，QCG-AgNPs在所研究的浓度范围内（3.125-100μg/ ml）显示出低毒性，并且纳米颗粒具有4个月的高稳定性（根据紫外线）。光谱数据）对表皮葡萄球菌的最高抗菌活性（3.91μg/ ml）。基于QCG衍生物生产AgNPs的高抗菌活性，稳定性和简便性表明，生产修饰的AgNPs的新方法值得进一步考虑。