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Receptor and signaling pathway involved in bovine lymphocyte activation by Atractylodis macrocephalae polysaccharides.
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2020-01-25 , DOI: 10.1016/j.carbpol.2020.115906 Wei Xu 1 , Sijia Fang 1 , Yong Wang 1 , Xiaoqing Chi 1 , Xiaodan Ma 1 , Tao Zhang 2 , Songhua Hu 1
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2020-01-25 , DOI: 10.1016/j.carbpol.2020.115906 Wei Xu 1 , Sijia Fang 1 , Yong Wang 1 , Xiaoqing Chi 1 , Xiaodan Ma 1 , Tao Zhang 2 , Songhua Hu 1
Affiliation
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The present study was to investigate the molecular mechanism underlying lymphocyte activation by total polysaccharides from Atractylodis macrocephalae (RAMPtp). The results showed that RAMPtp significantly promoted the secretions of cytokines (IFN-γ, IL-1α, IL-21, IFN-α, CCL4, CXCL9 and CXCL10), increased the proportions of CD4+ and CD8+ subpopulations, and enhanced the expressions of c-JUN, NFAT4, STAT1 and STAT3. microRNA sequencing identified 67 differentially expressed miRNAs (DEMs) in RAMPtp-stimulated SMLN lymphocytes, including 55 up-regulated and 12 down-regulated. GO and KEGG enrichment analyses of the predicted DEMs-targeted genes indicated that they were associated with immune system pathways, including PI3K-Akt, MAPKs, Jak-STAT and Calcium signaling pathways, which were confirmed by western blot and pathway inhibition assays. RAMPtp was further observed to favor immunostimulatory effect on both T and B lymphocytes via binding to TCR and membrane Ig individually. These findings might explain the immunomodulatory mechanism of RAMPtp in ameliorating the bovine intramammary infection.
中文翻译：
参与白头翁多糖激活牛淋巴细胞的受体和信号通路。
本研究旨在研究白术总多糖（RAMPtp）激活淋巴细胞的分子机制。结果表明，RAMPtp显着促进细胞因子（IFN-γ，IL-1α，IL-21，IFN-α，CCL4，CXCL9和CXCL10）的分泌，增加CD4 +和CD8 +亚群的比例，并增强c的表达-JUN，NFAT4，STAT1和STAT3。microRNA测序在RAMPtp刺激的SMLN淋巴细胞中鉴定出67个差异表达的miRNA（DEM），包括55个上调和12个下调。GO和KEGG对预测的DEM靶向基因的富集分析表明，它们与免疫系统途径相关，包括PI3K-Akt，MAPK，Jak-STAT和钙信号传导途径，这些均已通过Western印迹和途径抑制分析证实。进一步观察到RAMPtp通过分别与TCR和Ig膜结合，有利于T和B淋巴细胞的免疫刺激作用。这些发现可能解释了RAMPtp改善牛乳内感染的免疫调节机制。
更新日期：2020-01-26
中文翻译：
参与白头翁多糖激活牛淋巴细胞的受体和信号通路。
本研究旨在研究白术总多糖（RAMPtp）激活淋巴细胞的分子机制。结果表明，RAMPtp显着促进细胞因子（IFN-γ，IL-1α，IL-21，IFN-α，CCL4，CXCL9和CXCL10）的分泌，增加CD4 +和CD8 +亚群的比例，并增强c的表达-JUN，NFAT4，STAT1和STAT3。microRNA测序在RAMPtp刺激的SMLN淋巴细胞中鉴定出67个差异表达的miRNA（DEM），包括55个上调和12个下调。GO和KEGG对预测的DEM靶向基因的富集分析表明，它们与免疫系统途径相关，包括PI3K-Akt，MAPK，Jak-STAT和钙信号传导途径，这些均已通过Western印迹和途径抑制分析证实。进一步观察到RAMPtp通过分别与TCR和Ig膜结合，有利于T和B淋巴细胞的免疫刺激作用。这些发现可能解释了RAMPtp改善牛乳内感染的免疫调节机制。
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