H19 is a long non-coding RNA which was lowly expressed in chronic myeloid leukemia (CML). Here, we found that the overexpression of H19 significantly inhibited cell viability and colony formation and prolongs survival in CML cell lines and three xenografted mouse models. The H19 target proteins and microRNAs (miRNAs) were identified using a combination of computational prediction and RNA pull-down, including PCBP1, FUS protein, and miR-19a-3p and miR-106b-5p. Targeting PCBP1, FUS protein, miR-19a-3p, and miR-106b-5p significantly inhibits the cell growth and colony formation of CML cell lines. Co-overexpression of H19 and PCBP1, FUS, miR-19a-3p, and miR-106b-5p decreases the inhibitory effect of H19 in CML. These findings might provide a novel molecular insight into CML.

中文翻译：

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长链非编码RNA H19靶标的鉴定及其在慢性粒细胞白血病中的作用


H19是一种长链非编码RNA，在慢性粒细胞白血病（CML）中低表达。在这里，我们发现 H19 的过度表达显着抑制细胞活力和集落形成，并延长 CML 细胞系和三种异种移植小鼠模型的存活时间。采用计算预测和 RNA Pull-down 相结合的方式鉴定了 H19 靶蛋白和 microRNA (miRNA)，包括 PCBP1、FUS 蛋白以及 miR-19a-3p 和 miR-106b-5p。靶向 PCBP1、FUS 蛋白、miR-19a-3p 和 miR-106b-5p 显着抑制 CML 细胞系的细胞生长和集落形成。 H19 和 PCBP1、FUS、miR-19a-3p 和 miR-106b-5p 的共过表达会降低 H19 对 CML 的抑制作用。这些发现可能为 CML 提供新的分子视角。