A 66-year old mild cognitive impairment (MCI) female patient donated her Peripheral blood mononuclear cells (PBMC). PBMC was reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers in vitro. Moreover, the iPSC line displayed a normal karyotype. The apolipoprotein E (APOE) ε4 allele, is considered to be the strongest genetic risk factor for Sporadic Alzheimer's disease (AD). Our model might offer a good platform to study the pathological mechanisms and drug testing studies in AD and MCI.

一名66岁的轻度认知障碍（MCI）女性患者捐赠了她的外周血单个核细胞（PBMC）。使用包含重编程因子OCT4，KLF4，SOX2和C-MYC的非整合型仙台病毒载体对PBMC进行重编程。通过免疫细胞化学和体外自发分化为3个胚层的能力，证实了无转基因iPSC的多能性。此外，iPSC品系显示出正常的核型。载脂蛋白E（APOE）ε4等位基因被认为是偶发性阿尔茨海默氏病（AD）的最强遗传风险因素。我们的模型可能为研究AD和MCI中的病理机制和药物测试研究提供一个很好的平台。