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Cysteine Trisulfide Protects E. coli from Electrophile-Induced Death through the Generation of Cysteine Hydropersulfide.
Chemical Research in Toxicology ( IF 3.7 ) Pub Date : 2020-01-24 , DOI: 10.1021/acs.chemrestox.9b00494 Catherine F Henderson 1 , Iris Bica 1 , Faith T Long 1 , Drew D Irwin 1 , Christine H Stull 2 , Blaine W Baker 2 , Valeria Suarez Vega 2 , Zachary M Taugher 1 , Eliza D Fletes 1 , Juliet M Bartleson 1 , Megan L Humphrey 1 , Lucía Álvarez 3 , Masahiro Akiyama 4 , Yoshito Kumagai 4 , Jon M Fukuto 2 , Joseph Lin 1
Chemical Research in Toxicology ( IF 3.7 ) Pub Date : 2020-01-24 , DOI: 10.1021/acs.chemrestox.9b00494 Catherine F Henderson 1 , Iris Bica 1 , Faith T Long 1 , Drew D Irwin 1 , Christine H Stull 2 , Blaine W Baker 2 , Valeria Suarez Vega 2 , Zachary M Taugher 1 , Eliza D Fletes 1 , Juliet M Bartleson 1 , Megan L Humphrey 1 , Lucía Álvarez 3 , Masahiro Akiyama 4 , Yoshito Kumagai 4 , Jon M Fukuto 2 , Joseph Lin 1
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Hydropersulfide and polysulfide species have recently been shown to elicit a wide variety of biological and physiological responses. In this study, we examine the effects of cysteine trisulfide (Cys-SSS-Cys; also known as thiocystine) treatment on E. coli. Previous studies in mammalian cells have shown that Cys-SSS-Cys treatment results in protection from the electrophiles. Here, we show that the protective effect of Cys-SSS-Cys treatment against electrophile-induced cell death is conserved in E. coli. This protection correlates with the rapid generation of cysteine hydropersulfide (Cys-SSH) in the culture media. We go on to demonstrate that an exogenous phosphatase expressed in E. coli, containing only a single catalytic cysteine, is protected from electrophile-induced inactivation in the presence of hydropersulfides. These data together demonstrate that E. coli can utilize Cys-SSS-Cys to generate Cys-SSH and that the Cys-SSH can protect cellular thiols from reactivity with the electrophiles.
中文翻译：
半胱氨酸三硫化物可通过生成半胱氨酸氢过硫化物保护大肠杆菌免受亲电诱导的死亡。
最近已证明氢过硫化物和多硫化物物种引起广泛的生物学和生理反应。在这项研究中，我们检查了半胱氨酸三硫化物（Cys-SSS-Cys；也称为硫代胱氨酸）对大肠杆菌的影响。先前在哺乳动物细胞中的研究表明，Cys-SSS-Cys处理可防止亲电试剂。在这里，我们表明在大肠杆菌中，Cys-SSS-Cys治疗对亲电试剂诱导的细胞死亡的保护作用是保守的。这种保护与在培养基中快速生成半胱氨酸氢过氧化物（Cys-SSH）有关。我们继续证明，在大肠杆菌中表达的外源磷酸酶仅包含单个催化性半胱氨酸，在存在氢过硫化物的情况下可以防止亲电试剂诱导的失活。
更新日期：2020-01-31
中文翻译：
半胱氨酸三硫化物可通过生成半胱氨酸氢过硫化物保护大肠杆菌免受亲电诱导的死亡。
最近已证明氢过硫化物和多硫化物物种引起广泛的生物学和生理反应。在这项研究中，我们检查了半胱氨酸三硫化物（Cys-SSS-Cys；也称为硫代胱氨酸）对大肠杆菌的影响。先前在哺乳动物细胞中的研究表明，Cys-SSS-Cys处理可防止亲电试剂。在这里，我们表明在大肠杆菌中，Cys-SSS-Cys治疗对亲电试剂诱导的细胞死亡的保护作用是保守的。这种保护与在培养基中快速生成半胱氨酸氢过氧化物（Cys-SSH）有关。我们继续证明，在大肠杆菌中表达的外源磷酸酶仅包含单个催化性半胱氨酸，在存在氢过硫化物的情况下可以防止亲电试剂诱导的失活。
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