Systematic and Applied Microbiology ( IF 3.3 ) Pub Date : 2020-01-25 , DOI: 10.1016/j.syapm.2020.126064 Tatiana V Kochetkova 1 , Andrei V Mardanov 2 , Tatyana G Sokolova 1 , Elizaveta A Bonch-Osmolovskaya 3 , Ilya V Kublanov 1 , Vadim V Kevbrin 1 , Alexey V Beletsky 2 , Nikolay V Ravin 2 , Alexander V Lebedinsky 1
The ability to grow by anaerobic CO oxidation with production of H2 from water is known for some thermophilic bacteria, most of which belong to Firmicutes, as well as for a few hyperthermophilic Euryarchaeota isolated from deep-sea hydrothermal habitats. A hyperthermophilic, neutrophilic, anaerobic filamentous archaeon strain 1505 = VKM B-3180 = KCTC 15798 was isolated from a terrestrial hot spring in Kamchatka (Russia) in the presence of 30% CO in the gas phase. Strain 1505 could grow lithotrophically using carbon monoxide as the energy source with the production of hydrogen according to the equation CO + H2O → CO2 + H2; mixotrophically on CO plus glucose; and organotrophically on peptone, yeast extract, glucose, sucrose, or Avicel. The genome of strain 1505 was sequenced and assembled into a single chromosome. Based on 16S rRNA gene sequence analysis and in silico genome-genome hybridization, this organism was shown to be closely related to the Thermofilum adornatum species. In the genome of Thermofilum sp. strain 1505, a gene cluster (TCARB_0867-TCARB_0879) was found that included genes of anaerobic (Ni,Fe-containing) carbon monoxide dehydrogenase and genes of energy-converting hydrogenase ([Ni,Fe]-CODH–ECH gene cluster). Compared to the [Ni,Fe]-CODH–ECH gene clusters occurring in the sequenced genomes of other H2-producing carboxydotrophs, the [Ni,Fe]-CODH–ECH gene cluster of Thermofilum sp. strain 1505 presented a novel type of gene organization. The results of the study provided the first evidence of anaerobic CO oxidation coupled with H2 production performed by a crenarchaeon, as well as the first documented case of lithotrophic growth of a Thermofilaceae representative.
中文翻译:
能够通过厌氧一氧化碳氧化和H2产生而生长的第一只鱼眼。
某些嗜热细菌,其中大多数属于Firmicutes,以及从深海热液生境中分离出来的一些超嗜热Euryarchaeota,都知道通过厌氧CO氧化并从水中产生H 2来生长的能力。在气相中存在30%CO的情况下,从堪察加(俄罗斯)的地温泉中分离出了嗜热,嗜中性,厌氧的丝状古细菌菌株1505 = VKM B-3180 = KCTC 15798。根据一氧化碳CO + H 2 O→CO 2 + H 2,使用一氧化碳作为能源,菌株1505可以岩石状生长; 一氧化碳混合葡萄糖和葡萄糖;并且在蛋白ept,酵母提取物,葡萄糖,蔗糖或Avicel上有机营养。对菌株1505的基因组测序并组装成单个染色体。基于16S rRNA基因序列分析和计算机基因组-基因组杂交,该生物被证明与嗜热丝菌物种密切相关。在Thermofilum sp。的基因组中。发现该菌株为1505菌株,其基因簇(TCARB_0867-TCARB_0879)包含厌氧(含Ni,Fe的)一氧化碳脱氢酶基因和能量转换氢化酶的基因([Ni,Fe] -CODH–ECH基因簇)。与其他H 2的测序基因组中出现的[Ni,Fe] -CODH–ECH基因簇相比-生产carboxydotrophs时,[镍,铁]的-CODH-ECH基因簇Thermofilum藻。菌株1505提出了一种新型的基因组织。该研究的结果提供了第一个证据,证明厌氧的CO氧化和火锅鱼产生的H 2产生,以及第一个有记载的嗜热丝菌科植物石化生长的案例。