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Effect of Lactobacillus acidophilus D2/CSL (CECT 4529) supplementation in drinking water on chicken crop and caeca microbiome.
PLOS ONE ( IF 2.9 ) Pub Date : 2020-01-24 , DOI: 10.1371/journal.pone.0228338 Alessandra De Cesare 1 , Claudia Sala 2 , Gastone Castellani 2 , Annalisa Astolfi 3 , Valentina Indio 3 , Alberto Giardini 4 , Gerardo Manfreda 1
PLOS ONE ( IF 2.9 ) Pub Date : 2020-01-24 , DOI: 10.1371/journal.pone.0228338 Alessandra De Cesare 1 , Claudia Sala 2 , Gastone Castellani 2 , Annalisa Astolfi 3 , Valentina Indio 3 , Alberto Giardini 4 , Gerardo Manfreda 1
Affiliation
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In this study we gained insights into the effects of the supplementation with Lactobacillus acidophilus D2/CSL (CECT 4529) in the chicken drinking water on crop and caeca microbiomes. The probiotic was supplemented at the concentrations of 0.2 g Lactobacillus acidophilus/day/bird and 0.02 g Lactobacillus acidophilus/day/bird and its effect on the crop and caeca microbiomes was assessed at 14 and 35 days of rearing. The results showed that mean relative abundance of Lactobacillus acidophilus in the caeca did not show significative differences in the treated and control birds, although Lactobacillus acidophilus as well as Faecalibacterium prausnitzii, Lactobacillus crispatus and Lactobacillus reuteri significantly increased over time. Moreover, the treatment with the high dose of probiotic significantly increased the abundance of Clostridium asparagiforme, Clostridium hathewayi and Clostridium saccharolyticum producing butyrate and other organic acids supporting the chicken health. Finally, at 35 days, the Cell division protein FtsH (EC 3.4.24.-) and the Site-specific recombinase genes were significantly increased in the caeca of birds treated with the high dose of probiotic in comparison to the control group. The results of this study showed that Lactobacillus acidophilus D2/CSL (CECT 4529) supplementation in the drinking water at the concentrations of 0.2 and 0.02 g Lactobacillus acidophilus/day/bird improved beneficial microbes and functional genes in broiler crops and caeca. Nevertheless, the main site of action of the probiotic is the crop, at least in the early stage of the chicken life. Indeed, at 14 days Lactobacillus acidophilus was significantly higher in the crops of chickens treated with the high dose of LA in comparison to the control (14.094 vs 1.741%, p = 0.036).
中文翻译：
饮用水中添加嗜酸乳杆菌D2 / CSL（CECT 4529）对鸡肉和盲肠微生物组的影响。
在这项研究中，我们获得了在鸡饮用水中补充嗜酸乳杆菌D2 / CSL（CECT 4529）对作物和盲肠微生物群落的影响的见解。益生菌的添加浓度为0.2 g嗜酸乳杆菌/天/鸟和0.02 g嗜酸乳杆菌/天/鸟，并在饲养14天和35天时评估了其对农作物和盲肠微生物群的影响。结果表明，尽管嗜酸乳杆菌以及prausnitzii杆菌，crispatatus乳杆菌和reuteri乳杆菌随时间显着增加，但盲肠中嗜酸乳杆菌的平均相对丰度在处理和对照鸟类中没有显示出显着差异。此外，高剂量益生菌的治疗显着提高了天门氏梭菌，梭状梭状芽孢杆菌和解糖梭状芽孢杆菌的丁酸和其他有机酸的含量，从而提高了鸡肉的健康。最后，在第35天时，与对照组相比，用高剂量益生菌治疗的家禽的盲肠中的细胞分裂蛋白FtsH（EC 3.4.24.-）和位点特异性重组酶基因显着增加。这项研究的结果表明，在饮用水中添加嗜酸乳杆菌D2 / CSL（CECT 4529）的浓度为0.2和0.02 g /天/只鸟，可改善肉鸡作物和盲肠中的有益微生物和功能基因。不过，益生菌的主要作用部位是农作物，至少在鸡的生命早期。实际上，与对照相比，在用高剂量的LA处理的鸡的作物中，第14天的嗜酸乳杆菌明显更高（14.094对1.741％，p = 0.036）。
更新日期：2020-01-26
中文翻译：
饮用水中添加嗜酸乳杆菌D2 / CSL（CECT 4529）对鸡肉和盲肠微生物组的影响。
在这项研究中，我们获得了在鸡饮用水中补充嗜酸乳杆菌D2 / CSL（CECT 4529）对作物和盲肠微生物群落的影响的见解。益生菌的添加浓度为0.2 g嗜酸乳杆菌/天/鸟和0.02 g嗜酸乳杆菌/天/鸟，并在饲养14天和35天时评估了其对农作物和盲肠微生物群的影响。结果表明，尽管嗜酸乳杆菌以及prausnitzii杆菌，crispatatus乳杆菌和reuteri乳杆菌随时间显着增加，但盲肠中嗜酸乳杆菌的平均相对丰度在处理和对照鸟类中没有显示出显着差异。此外，高剂量益生菌的治疗显着提高了天门氏梭菌，梭状梭状芽孢杆菌和解糖梭状芽孢杆菌的丁酸和其他有机酸的含量，从而提高了鸡肉的健康。最后，在第35天时，与对照组相比，用高剂量益生菌治疗的家禽的盲肠中的细胞分裂蛋白FtsH（EC 3.4.24.-）和位点特异性重组酶基因显着增加。这项研究的结果表明，在饮用水中添加嗜酸乳杆菌D2 / CSL（CECT 4529）的浓度为0.2和0.02 g /天/只鸟，可改善肉鸡作物和盲肠中的有益微生物和功能基因。不过，益生菌的主要作用部位是农作物，至少在鸡的生命早期。实际上，与对照相比，在用高剂量的LA处理的鸡的作物中，第14天的嗜酸乳杆菌明显更高（14.094对1.741％，p = 0.036）。
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