The cell wall of filamentous fungi, comprised of chitin, polysaccharide and glycoproteins, maintains the integrity of hyphae and protect them from defence responses by potential host plants. Here, we report that one polysaccharide deacetylase of Puccinia striiformis f. sp. tritici (Pst ), Pst_13661, suppresses Bax‐induced cell death in plants and Pst_13661 is highly induced during early stages of the interaction between wheat and Pst . Importantly, the transgenic wheat expressing the RNA interference (RNAi) construct of Pst_13661 exhibits high resistance to major Pst epidemic races CYR31, CYR32 and CYR33 by inhibiting growth and development of Pst , indicating that Pst_13661 is an available pathogenicity factor and is a potential target for generating broad‐spectrum resistance breeding material of wheat. It forms a homo‐polymer and has high affinity for chitin and germ tubes of Pst compared with the control. Besides, Pst_13661 suppresses chitin‐induced plant defence in plants. Hence, we infer that Pst_13661 may modify the fungal cell wall to prevent recognition by apoplastic surveillance systems in plants. This study opens new approaches for developing durable disease‐resistant germplasm by disturbing the growth and development of fungi and develops novel strategies to control crop diseases.

中文翻译：

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一种来自Puccinia striiformis f。的多糖脱乙酰酶。sp。小麦是抑制植物免疫力的重要致病基因。

丝质真菌的细胞壁由几丁质，多糖和糖蛋白组成，可以保持菌丝的完整性，并保护它们免受潜在寄主植物的防御反应。在这里，我们报道了一种Puccinia striiformis f的多糖脱乙酰酶。sp。小麦（的Pst），Pst_13661，抑制Bax蛋白诱导的细胞死亡在植物和Pst_13661期间小麦和之间的相互作用的早期阶段高度诱导的Pst。重要的是，表达Pst_13661的RNA干扰（RNAi）构建体的转基因小麦通过抑制Pst的生长和发育对主要的Pst流行种族CYR31，CYR32和CYR33表现出高抗性，表明Pst_13661是可利用的致病因子，并且是产生小麦广谱抗性育种材料的潜在目标。与对照相比，它形成均聚物，对Pst的几丁质和胚芽管具有高亲和力。此外，Pst_13661抑制了几丁质诱导的植物防御。因此，我们推断Pst_13661可能会修饰真菌细胞壁，以防止植物中质外体监测系统的识别。这项研究通过干扰真菌的生长和发育为开发持久的抗病种质开辟了新途径，并开发了控制作物病害的新策略。