A previous study reported that exposure to tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) could promote the progression of hepatocellular carcinoma (HCC) in female HCC model zebrafish. Due to the existence of gender disparity in the development of HCC between females and males, whether the promotion effect of TDCIPP still exists in male HCC model zebrafish remains unclear. In this study, Tg(fabp10:rtTA2s-M2; TRE2:EGFP-krasG12V), referred as kras transgenic zebrafish which was shown to be an inducible liver tumor model, was applied as experimental model to assess the promotion potential of TDCIPP for HCC in males. In brief, kras males were exposed to 20 mg/L doxycycline (DOX), 0.3 mg/L TDCIPP and a binary mixture of 20 mg/L DOX with 0.3 mg/L TDCIPP, and after exposure liver size, histopathology and transcriptional profiles of liver from these treatments were examined. With the involvement of TDCIPP, the liver size was significantly increased and the lesion of hepatocyte became more aggressive. Furthermore, expressions of genes involved in DNA replication and inflammatory response were simultaneously up-regulated in the treatment of TDCIPP compared with the solvent control and in the treatment of the binary mixture of the two chemicals compared to the single DOX treatment. Overall, our results suggested that TDCIPP had promotion effect on the progression of liver tumor in kras males.

中文翻译：

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磷酸三（1,3-二氯-2-丙基）酯诱导的雄性克拉斯转基因斑马鱼肝肿瘤进展的促进作用。

先前的一项研究报道，暴露于磷酸三（1,3-二氯-2-丙基）酯（TDCIPP）可以促进雌性HCC模型斑马鱼的肝细胞癌（HCC）进展。由于雌雄之间在HCC发育中存在性别差异，因此尚不清楚TDCIPP的促进作用是否仍存在于雄性HCC模型斑马鱼中。在这项研究中，Tg（fabp10：rtTA2s-M2; TRE2：EGFP-krasG12V）被称为kras转基因斑马鱼，它被证明是可诱导的肝肿瘤模型，被用作实验模型评估TDCIPP对HCC的促进潜力男性。简而言之，kras雄性动物暴露于20 mg / L强力霉素（DOX），0.3 mg / L TDCIPP和20 mg / L DOX与0.3 mg / L TDCIPP的二元混合物，暴露后的肝脏大小 检查了来自这些治疗的肝脏的组织病理学和转录谱。随着TDCIPP的参与，肝脏的大小明显增加，肝细胞的病变变得更具侵略性。此外，与溶剂对照相比，在TDCIPP的处理中和与单一DOX处理相比，在两种化学物质的二元混合物的处理中，涉及DNA复制和炎症反应的基因的表达同时上调。总体而言，我们的结果表明TDCIPP对kras男性的肝肿瘤进展具有促进作用。与溶剂对照相比，在TDCIPP处理中和与单一DOX处理相比，两种化学物质的二元混合物处理中，涉及DNA复制和炎症反应的基因的表达同时上调。总体而言，我们的结果表明TDCIPP对kras男性的肝肿瘤进展具有促进作用。与溶剂对照相比，在TDCIPP处理中和与单一DOX处理相比，两种化学物质的二元混合物处理中，涉及DNA复制和炎症反应的基因的表达同时上调。总体而言，我们的结果表明TDCIPP对kras男性的肝肿瘤进展具有促进作用。