The potential public health impact of foodborne parasites transmitted via contaminated fresh produces indicates the necessity for robust and reliable laboratory methods for their detection and identification on this infection vehicle. Standardization of methods for detection of common FBP in fresh produce is to be expected and ensuring that the DNA extraction approach is most appropriate for the FBP of interest and for the matrix being analyzed is also important. Therefore, the aim of the present study was to compare the efficacy of two commercially available DNA extraction procedures, the UNEX-based method and DNeasy PowerSoil kit in the detection of three protozoan parasites, C. cayetanensis, C. parvum, and T. gondii, on contaminated berries. Oocysts of each parasite were spiked into the pellets of raspberry and blueberry washes. The spiked pellets were then randomly assigned to DNA extraction using either the PowerSoil or UNEX method, with DNA extraction with both methods performed by two independent analysts. The detection rate when berry washes were spiked with 20 oocysts of C. cayetanensis, T. gondii, and C. parvum was 95%, 85%, and 40%, respectively, when using the PowerSoil kit; whereas the equivalent results using the UNEX method were 55%, 60%, and 5%, respectively. In addition, significantly lower C_q values were achieved for each parasite in the samples spiked with 500 oocysts when the PowerSoil kit was used. Possible reasons for these results are discussed, and include the composition of both the beads and the buffers in each method.

通过污染的新鲜农产品传播的食源性寄生虫对公共卫生的潜在影响表明，有必要采用健壮可靠的实验室方法在这种感染媒介上进行检测和鉴定。新鲜农产品中常见FBP检测方法的标准化有望实现，确保DNA提取方法最适合目标FBP和所分析的基质也很重要。因此，本研究的目的是比较两种市售DNA提取程序，基于UNEX的方法和DNeasy PowerSoil试剂盒在检测三种原生动物寄生虫（C. cayetanensis，C。parvum和T. gondii）中的功效。，在受污染的浆果上。将每种寄生虫的卵囊掺入覆盆子和蓝莓洗涤的颗粒中。然后使用PowerSoil或UNEX方法将加标的沉淀物随机分配到DNA提取中，两种方法都由两名独立的分析人员进行DNA提取。当使用PowerSoil试剂盒时，将浆果洗净液掺入20个美国大隐孢子虫，弓形虫和小球藻的卵囊中时的检出率分别为95％，85％和40％。而使用UNEX方法的等效结果分别为55％，60％和5％。此外，C _q大大降低使用PowerSoil试剂盒时，每个样品中掺有500个卵囊的寄生虫均获得了最高值。讨论了产生这些结果的可能原因，包括每种方法中珠子和缓冲液的组成。