Extraction, purification, characterization, and rheological properties of a glycoprotein from Cynomorium songaricum Rupr,Biotechnology and Applied Biochemistry

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Extraction, purification, characterization, and rheological properties of a glycoprotein from Cynomorium songaricum Rupr
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-01-25 , DOI: 10.1002/bab.1892
Jipeng Shi _{1,

2} , Yunfei Xu _{1,

2} , Jie Guo _{1,

2} , Xingxing Mu _{1,

2} , Chengbo Wang _{1,

2} , Xin Chen _{1,

2} , Ji Zhang _{1,

2}

Affiliation

Cynomorium songaricum Rupr is widely known in China as a traditional herbal medicine. In this study, single‐factor experiments and response surface methodology were used to optimize the extraction of Cynomorium songaricum Rupr glycoprotein (CSG). The results show that a maximum glycoprotein yield of 6.39 ± 0.32% was achieved at a ratio of solid to liquid 32:1 for 4.2 H at 52 °C. Then, the IR, monosaccharide composition, amino acid composition, type of glycopeptide linkage, and average molecular weight of CSG‐1 purified from CSG were characterized. The results indicate that CSG‐1 presented the characteristic absorption peak of polysaccharide and protein, including four monosaccharides and 17 amino acids, had O‐linked glycopeptide bonds, M_w, W_n, M_w/M_n, M_p, and the z‐average were 5.343 × 10⁶, 3.203 × 10⁶, 1.668, 8.911 × 10⁶, and 6.948 × 10⁶, respectively. Besides, CSG‐1 solution was described by the Herschel–Bulkley model and it behaved as a shear‐thinning fluid. Also, under a frequency sweep the moduli G′ and G″ both increased with increasing CSG‐1 concentration and the CSG‐1 dispersions had weak thermal stability over the temperature sweep. These results provide a scientific basis for the further study of Cynomorium songaricum Rupr.

更新日期：2020-01-25

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