We introgressed wheatgrass germplasm from the octoploid amphiploid Triticum aestivum× Lophopyrum elongatum into wheat by manipulating the wheat Ph1 gene and discovered and characterized 130 introgression lines harboring single or, in various combinations, complete and recombined L. elongatum chromosomes. Diploid wheatgrass Lophopyrum elongatum (genomes EE) possesses valuable traits for wheat genetics and breeding. We evaluated several strategies for introgression of this germplasm into wheat. To detect it, we developed and validated multiplexed sets of Sequenom MassARRAY single nucleotide polymorphism (SNP) markers, which differentiated disomic and monosomic L. elongatum chromosomes from wheat chromosomes. We identified 130 introgression lines (ILs), which harbored 108 complete and 89 recombined L. elongatum chromosomes. Of the latter, 59 chromosomes were recombined by one or more crossovers and 30 were involved in centromeric (Robertsonian) translocations or were telocentric. To identify wheat chromosomes substituted for or recombined with L. elongatum chromosomes, we genotyped the ILs with the wheat 90-K Infinium SNP array. We found that most of the wheat 90-K probes correctly detected their targets in the L. elongatum genome and showed that some wheat SNPs are ancient and had originated prior to the divergence of the wheat and L. elongatum lineages. Of the 130 ILs, 52% were homozygous for Ph1 deletion and thus are staged to be recombined further. We failed to detect in the L. elongatum genome the 4/5 reciprocal translocation that has been reported in Thinopyrum bessarabicum and several other Triticeae genomes.

中文翻译：

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从面包小麦 × Lophopyrum elongatum 双倍体到小麦的全基因组渗入。

我们通过操纵小麦 Ph1 基因将八倍体双倍体 Triticum aestivum × Lophopyrum elongatum 的小麦草种质渗入小麦中，发现并表征了 130 个含有单个或各种组合的完整和重组 L. elongatum 染色体的渗入品系。二倍体小麦草 Lophopyrum elongatum (基因组 EE) 具有对小麦遗传和育种有价值的性状。我们评估了几种将这种种质渗入小麦的策略。为了检测它，我们开发并验证了多路复用的 Sequenom MassARRAY 单核苷酸多态性 (SNP) 标记集，这些标记将二体和单体 L. elongatum 染色体与小麦染色体区分开来。我们确定了 130 个渐渗系 (ILs)，其中包含 108 条完整和 89 条重组 L. elongatum 染色体。后者中，59 条染色体通过一次或多次交叉重组，30 条涉及着丝粒（罗伯逊）易位或端着丝粒。为了鉴定替代或重组 L. elongatum 染色体的小麦染色体，我们用小麦 90-K Infinium SNP 阵列对 ILs 进行基因分型。我们发现，大多数小麦 90-K 探针在 L. elongatum 基因组中正确检测到它们的目标，并表明一些小麦 SNP 是古老的，并且起源于小麦和 L. elongatum 谱系的分歧之前。在 130 个 IL 中，52% 是 Ph1 缺失的纯合子，因此被分阶段进行进一步重组。我们未能在 L. elongatum 基因组中检测到已在 Thinopyrum bessarabicum 和其他几个小麦科基因组中报道的 4/5 相互易位。