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Quick and Cost-Effective Estimation of Vitamin C in Multifruit Juices using Voltammetric Methods.
Sensors ( IF 3.4 ) Pub Date : 2020-01-26 , DOI: 10.3390/s20030676
Jose-Antonio López-Pastor 1 , Ascensión Martínez-Sánchez 2, 3 , Juan Aznar-Poveda 1 , Antonio-Javier García-Sánchez 1 , Joan García-Haro 1 , Encarnación Aguayo 2, 3
Affiliation  

Ascorbic Acid (AA) is a natural and powerful water-soluble antioxidant associated with long-lasting food products. As time passes, the AA content in products sharply decreases, and they become increasingly degraded. There are several techniques to precisely quantify AA concentrations. However, most of them employ costly laboratory instruments, such as High-Performance Liquid Chromatography (HPLC) or complex electrochemical methods, which make unfeasible recurrent AA measurements along the entire supply chain. To address this issue, we contribute with an in-field and real-time voltammetric method, carried out with a low-cost, easy-to-use, and portable device. An unmodified Screen-Printed Electrode (SPE) is used together with the device to achieve short reading times. Our method has been extensively tested in two multifruit juices using three different SPEs. Calibration curves and Limit of Detection were derived for each SPE. Furthermore, periodic experiments were conducted to study the shelf life of juices under consideration. During the analysis, a set of assays for each SPE were implemented to determine the remaining AA amount per juice and compare it with that obtained using HPLC under the same conditions. Results revealed that our cost-effective device is fully comparable to the HPLC equipment, as long as the juice does not include certain interferents; a scenario also contemplated in this article.

中文翻译:

使用伏安法快速,经济高效地估算果汁中的维生素C。

抗坏血酸(AA)是一种天然且强大的水溶性抗氧化剂,可与持久食品相关联。随着时间的流逝,产品中的AA含量急剧下降,并且降解程度越来越高。有几种技术可以精确定量AA浓度。但是,它们中的大多数都采用了昂贵的实验室仪器,例如高效液相色谱(HPLC)或复杂的电化学方法,这些方法无法在整个供应链中进行反复的AA测量。为了解决这个问题,我们提供了一种现场,实时伏安法,并采用了一种低成本,易于使用的便携式设备。未经修改的丝网印刷电极(SPE)与设备一起使用可缩短读取时间。我们的方法已经在使用三种不同SPE的两种多汁果汁中进行了广泛的测试。为每个SPE导出了校正曲线和检出限。此外,进行了定期实验以研究所考虑的果汁的保质期。在分析过程中,对每种SPE进行了一套测定,以确定每个果汁中的剩余AA量,并将其与在相同条件下使用HPLC获得的AA量进行比较。结果表明,只要果汁中不包含某些干扰物,我们的高性价比设备就可以与HPLC设备完全媲美。本文还考虑了一种方案。对每种SPE进行了一系列测定,以确定每个果汁中的剩余AA量,并将其与在相同条件下使用HPLC获得的AA量进行比较。结果表明,只要果汁中不包含某些干扰物,我们的高性价比设备就可以与HPLC设备完全媲美。本文还考虑了一种方案。对每种SPE进行了一系列测定,以确定每个果汁中的剩余AA量,并将其与在相同条件下使用HPLC获得的AA量进行比较。结果表明,只要果汁中不包含某些干扰素,我们的高性价比设备就可以与HPLC设备完全媲美。本文还考虑了一种方案。
更新日期:2020-01-26
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