Noninvasive blood glucose monitoring has been a long-standing dream in diabetes management. The use of Raman spectroscopy, with its molecular specificity, has been investigated in this regard over the past decade. Previous studies reported on glucose sensing based on indirect evidence such as statistical correlation to the reference glucose concentration. However, these claims fail to demonstrate glucose Raman peaks, which has raised questions regarding the effectiveness of Raman spectroscopy for glucose sensing. Here, we demonstrate the first direct observation of glucose Raman peaks from in vivo skin. The signal intensities varied proportional to the reference glucose concentrations in three live swine glucose clamping experiments. Tracking spectral intensity based on linearity enabled accurate prospective prediction in within-subject and intersubject models. Our direct demonstration of glucose signal may quiet the long debate about whether glucose Raman spectra can be measured in vivo in transcutaneous glucose sensing.

中文翻译：

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使用体内拉曼光谱法直接观察葡萄糖指纹。

无创血糖监测一直是糖尿病管理中的长期梦想。在过去的十年中，已经在这方面研究了具有分子特异性的拉曼光谱法的使用。先前的研究报道了基于间接证据（例如与参考葡萄糖浓度的统计相关性）的葡萄糖感测。然而，这些主张未能证明葡萄糖拉曼峰，这引起了关于拉曼光谱法用于葡萄糖感测的有效性的疑问。在这里，我们展示了首次从体内皮肤直接观察到葡萄糖拉曼峰。在三个活猪葡萄糖钳制实验中，信号强度与参考葡萄糖浓度成比例变化。基于线性度跟踪光谱强度可以在对象内和对象间模型中进行准确的前瞻性预测。我们对葡萄糖信号的直接展示可能使人们对是否可以在经皮葡萄糖感测中体内测量葡萄糖拉曼光谱的争论变得平静。