Telomere anchoring to nuclear envelope (NE) is a key feature of nuclear genome architecture. Peripheral localization of telomeres is important for chromatin silencing, telomere replication and for the control of inappropriate recombination. Here, we report that fission yeast quiescent cells harbor predominantly a single telomeric cluster anchored to the NE. Telomere cluster association to the NE relies on Rap1-Bqt4 interaction, which is impacted by the length of telomeric sequences. In quiescent cells, reducing telomere length or deleting bqt4, both result in an increase in transcription of the telomeric repeat-containing RNA (TERRA). In the absence of Bqt4, telomere shortening leads to deep increase in TERRA level and the concomitant formation of subtelomeric rearrangements (STEEx) that accumulate massively in quiescent cells. Taken together, our data demonstrate that Rap1-Bqt4-dependent telomere association to NE preserves telomere integrity in post-mitotic cells, preventing telomeric transcription and recombination. This defines the nuclear periphery as an area where recombination is restricted, creating a safe zone for telomeres of post-mitotic cells.

中文翻译：

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端粒的核被膜附着限制了静态裂变酵母细胞中的TERRA和端粒重排。

端粒锚定到核膜（NE）是核基因组结构的关键特征。端粒的周边定位对于染色质沉默，端粒复制和不适当重组的控制很重要。在这里，我们报告裂变酵母静态细胞主要包含锚定到NE的单个端粒簇。端粒簇与NE的结合依赖于Rap1-Bqt4相互作用，该相互作用受端粒序列长度的影响。在静态细胞中，减少端粒长度或删除bqt4都会导致含端粒重复序列的RNA（TERRA）的转录增加。在缺少Bqt4的情况下，端粒缩短会导致TERRA水平的大幅增加，并伴随着大量形成于静止细胞中的亚端粒重排（STEEx）的形成。在一起 我们的数据表明，依赖于Rap1-Bqt4的端粒与NE的结合保留了有丝分裂后细胞中的端粒完整性，从而防止了端粒的转录和重组。这将核外围定义为限制重组的区域，从而为有丝分裂后细胞的端粒创造了一个安全区域。