Lymantria dispar asiatica is a globally distributed herbivorous pest. Avermectin is a highly effective, broad-spectrum insecticide. In this study, fourth instar L. dispar asiatica larvae were exposed to a LC30 dose of avermectin. The structure and function of larval gut microbial community was analyzed to examine how gut microbiota in L. dispar asiatica larvae responded to avermectin stress. Results showed that the structure and function of gut microbial community in L. dispar asiatica larvae were varied by avermectin stress. To be precise, more than half quantity of the observed Optical Taxonomic Units (OTUs) showed significantly different abundances under avermectin stress. Linear discriminant analysis effect size (LEfSe) suggested nine bacterial genera and 12 fungal genera contributed to the different gut microbial community structure in L. dispar asiatica larvae. Gut microbial function classification (PICRUSt and FUNGuild) suggested that three bacterial function categories and a fungal function guild were significantly increased, and two fungal function guilds were significantly decreased by avermectin stress. This study furthers our understanding of the physiology of L. dispar asiatica larvae under avermectin stress, and is an essential step towards future development of potential pesticide targets.

中文翻译：

---

阿维菌素胁迫改变了 Lymantria dispar asiatica (Lepidoptera: Lymantriidae) 幼虫肠道微生物群落的结构和功能

Lymantria dispar asiatica 是一种全球分布的草食性害虫。阿维菌素是一种高效、广谱的杀虫剂。在这项研究中，四龄 L. dispar asiatica 幼虫暴露于 LC30 剂量的阿维菌素。分析了幼虫肠道微生物群落的结构和功能，以检查 L. dispar asiatica 幼虫的肠道微生物群如何响应阿维菌素胁迫。结果表明，阿维菌素胁迫改变了L. dispar asiatica幼虫肠道微生物群落的结构和功能。准确地说，观察到的光学分类单位 (OTU) 中有一半以上在阿维菌素胁迫下显示出显着不同的丰度。线性判别分析效应大小 (LEfSe) 表明 9 个细菌属和 12 个真菌属促成了 L. 中不同的肠道微生物群落结构。dispar asiatica 幼虫。肠道微生物功能分类（PICRUSt 和 FUNGuild）表明，阿维菌素胁迫显着增加了三个细菌功能类别和一个真菌功能类别，而两个真菌功能类别显着降低。这项研究进一步加深了我们对阿维菌素胁迫下 L. dispar asiatica 幼虫生理的理解，并且是未来开发潜在农药目标的重要一步。