Abstract As foodborne pathogen contamination has become a threat to human health worldwide, early detection of pathogen is significant. Traditional electrochemical immunosensors for pathogen detection involve electrode modification or samples adsorption on the electrode which affect the detection sensitivity and electrode reproducibility, thereby limiting their further application in POCT (point of care testing). We established a homogeneous magnetic bead-based electrochemical impedance system using interdigitated gold electrodes as sensing elements for high sensitivity detection of Escherichia coli (E.coli) O157:H7. The system avoided electrode modification and sample matrix adsorption, and greatly improved detection sensitivity. The detection limit of this method for E.coli O157:H7 was 102 colony-forming units per milliliter (CFU mL−1), coefficient of variation (CV)

中文翻译：

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一种用于高灵敏度检测大肠杆菌 O157:H7 的均质磁珠阻抗免疫传感器

摘要 由于食源性病原体污染已成为全球范围内对人类健康的威胁，病原体的早期检测具有重要意义。传统的用于病原体检测的电化学免疫传感器涉及电极修饰或样品吸附在电极上，这会影响检测灵敏度和电极重现性，从而限制了它们在 POCT（即时检测）中的进一步应用。我们建立了一个基于均质磁珠的电化学阻抗系统，使用交叉金电极作为传感元件，用于大肠杆菌（E.coli）O157:H7 的高灵敏度检测。该系统避免了电极修饰和样品基质吸附，大大提高了检测灵敏度。该方法对大肠杆菌 O157:H7 的检测限为每毫升 102 个菌落形成单位 (CFU mL-1)，