Abstract The present study optimized ethanol yield using nickel oxide (NiO) nanoparticles (NPs) as a biocatalyst. Additionally, Saccharomyces cerevisiae BY4743 cell growth and the bioethanol production kinetics were assessed. The Response Surface Methodology (RSM) model showed a coefficient of determination (R2) value of 0.93. The optimized process gave a biomass concentration and ethanol yield of 2.04 g/L and 0.26 g/g (1.03 and 1.19-fold increment compared to the control experiment), respectively. The process kinetic data showed that the inclusion of NiO NPs improved the affinity of S. cerevisiae BY4743 to glucose consumption, carbohydrate and protein accumulation. A significant reduction in volatile fatty acid (VFA) was observed in the presence of NiO NPs. The application of nano biocatalyst in simultaneous saccharification and fermentation of potato peel waste, meaningfully enhanced bioethanol production (>65 %). The study provided major insights into the use of NiO NPs to enhance the bioprocess of ethanol production.

中文翻译：

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氧化镍纳米颗粒对生物乙醇生产的影响：工艺优化、动力学和代谢研究

摘要 本研究使用氧化镍 (NiO) 纳米粒子 (NP) 作为生物催化剂优化乙醇产量。此外，还评估了酿酒酵母 BY4743 细胞生长和生物乙醇生产动力学。响应面方法 (RSM) 模型显示的决定系数 (R2) 值为 0.93。优化过程的生物质浓度和乙醇产量分别为 2.04 g/L 和 0.26 g/g（与对照实验相比增加了 1.03 和 1.19 倍）。过程动力学数据表明，NiO NPs 的加入提高了酿酒酵母 BY4743 对葡萄糖消耗、碳水化合物和蛋白质积累的亲和力。在 NiO NPs 的存在下观察到挥发性脂肪酸 (VFA) 的显着减少。纳米生物催化剂在马铃薯皮废料同步糖化发酵中的应用，显着提高了生物乙醇的产量（>65%）。该研究为使用 NiO NP 增强乙醇生产的生物过程提供了重要见解。