Abstract Symbiotic plant-microbe metabolic interactions not only have beneficial effects on plants but also contribute to rich, unmatched and complex chemical biodiversity with biological potential. Systematic delineated bioprospecting of fungal diversity associated with Ficus pumila Linn (Moraceae) for antimicrobial metabolite revealed Xylaria sp. FPL-25(M). The present study describes bioactivity guided fractionation prioritized for antimicrobial potential. Thus, chemical investigation of culture broth of Xylaria sp. FPL-25(M) by bioactivity guided fractionation with spectroscopic techniques revealed bioactive metabolite xylobovide-9-methyl ester. The xylobovide-9-methyl ester exhibited broad-spectrum antimicrobial activity. However, Gram-positive bacteria and fungi were more susceptible than Gram-negative bacteria. The present study results represent bioassay-based screening strategy which facilitates rapid, efficient and reliable approach for endophytic strain prioritization for novel bioactive molecules.

中文翻译：

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木霉属抗菌代谢物的生物活性引导分离

摘要 共生植物-微生物代谢相互作用不仅对植物有益，而且有助于丰富、无与伦比和复杂的具有生物潜力的化学生物多样性。与 Ficus pumila Linn（桑科）相关的真菌多样性的系统描绘生物勘探用于抗菌代谢物揭示了 Xylaria sp。FPL-25(M)。本研究描述了优先考虑抗菌潜力的生物活性引导分馏。因此，Xylaria sp. 培养液的化学研究。FPL-25(M) 通过生物活性引导分馏和光谱技术揭示了生物活性代谢物 xylobovide-9-methyl 酯。xylobvidide-9-methyl 酯表现出广谱抗菌活性。然而，革兰氏阳性菌和真菌比革兰氏阴性菌更易感。