Migration of the macrophages to the injured site soon after the skeletal muscle injury is crucial for subsequent regeneration of the muscle fibers. The Monocyte chemoattractant protein-1 (MCP-1) is important chemokine for regulating migration of the monocytes/macrophages. Earlier reports have discussed that icing applied soon after muscle crush injury retards muscle regeneration through retardation of macrophage migration. The MCP-1+ cells and neutrophils might promote the migration of the macrophages. To test the hypothesis that icing soon after the skeletal muscle injury affects MCP-1+ cells and neutrophils, we examined the effect of icing on MCP-1+ cells and neutrophils after crush injury to skeletal muscle in rats. Owing to the icing application for 20 min soon after the injury, accumulation of the macrophages was inhibited until 12 h after injury. Numbers of the neutrophils at 3 h after the injury and the MCP-1+ cells at 6 h and later after the injury in the icing group were significantly lower than those in the non-icing group, suggesting that these phenomena contribute to the retardation of macrophage migration.

中文翻译：

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在结冰损伤大鼠骨骼肌后不久应用结冰抑制MCP-1阳性细胞的迁移。

骨骼肌损伤后巨噬细胞向损伤部位的迁移对于随后的肌纤维再生至关重要。单核细胞趋化蛋白-1（MCP-1）是调节单核细胞/巨噬细胞迁移的重要趋化因子。较早的报道已经讨论过，在肌肉挤压伤后不久应用结冰会通过阻止巨噬细胞迁移来阻止肌肉再生。MCP-1 +细胞和嗜中性粒细胞可能会促进巨噬细胞的迁移。为了检验假说骨骼肌损伤后结冰会影响MCP-1 +细胞和中性粒细胞，我们检查了结冰对大鼠骨骼肌挤压损伤后对MCP-1 +细胞和中性粒细胞的影响。由于受伤后不久要结冰20分钟，巨噬细胞的聚集被抑制直到受伤后12小时。结冰组3h损伤后中性粒细胞的数目以及损伤后6h及以后损伤后的MCP-1 +细胞的数目均显着低于非结冰组，这表明这些现象有助于抑制结冰。巨噬细胞迁移。