Isothermal amplification, such as loop-mediated nucleic acid isothermal amplification (LAMP), is a highly promising technique that could revolutionize portable or point-of-care gene detection. However, the commonly used readouts of these isothermal reactions have been limited to a small number of options such as real-time fluorescence and colorimetric paper strips, which suffer from practical difficulties in further integration and quantitation, respectively. To enrich the readout library and provide more options suitable for a variety of detection requirements, we report a ready-to-use gene testing method based on the execution of isothermal nucleic acid amplification reactions on closed and portable PGE-LAMP electrochemical chips. Taking the HF183 gene of the fecal pollutant Bacteroides as a model target, this method allows both end-point and real-time transduction from genomic information to electrochemical signals with ultra-high sensitivity, specificity and a good signal-to-noise ratio, and with a detection limit as low as 80 copies. This approach is therefore highly promising for the development of point-of-care detection systems and accelerating the practical application of LAMP.

等温扩增，例如环介导的核酸等温扩增（LAMP），是一种很有前途的技术，可以彻底改变便携式或即时检测基因的检测方法。但是，这些等温反应的常用读数仅限于少数几种选择，例如实时荧光和比色纸条，它们分别在进一步的积分和定量方面存在实际困难。为了丰富读出库并提供适合各种检测要求的更多选项，我们报告了一种在封闭式和便携式PGE-LAMP电化学芯片上基于等温核酸扩增反应执行的即用型基因测试方法。粪便中拟杆菌的HF183基因作为模型目标，此方法可实现从基因组信息到电化学信号的端点和实时转导，具有超高的灵敏度，特异性和良好的信噪比，并且检测限低至80个拷贝。因此，这种方法对于即时诊断系统的开发和加速LAMP的实际应用是非常有前途的。