Interleukin-4 Gene Transfection and Spheroid Formation Potentiate Therapeutic Efficacy of Mesenchymal Stem Cells for Osteoarthritis.,Advanced Healthcare Materials

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Interleukin-4 Gene Transfection and Spheroid Formation Potentiate Therapeutic Efficacy of Mesenchymal Stem Cells for Osteoarthritis.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-01-24 , DOI: 10.1002/adhm.201901612
Seuk Young Song ₁ , Jihye Hong ₂ , Seukhyeong Go ₂ , Songhyun Lim ₁ , Hee Su Sohn ₁ , Mikyung Kang ₂ , Gun-Jae Jung ₁ , Jeong-Kee Yoon ₁ , Mi Lan Kang ₃ , Gun-Il Im ₃ , Byung-Soo Kim _{1,

2,

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Affiliation

Osteoarthritis (OA) is a painful intractable disease that significantly affects patients' quality of life. However, current therapies, such as pain killers and joint replacement surgery, do not lead to cartilage protection. Mesenchymal stem cells (MSCs) have been proposed as an alternative strategy for OA therapy because MSCs can secrete chondroprotective and anti-inflammatory factors. However, interleukin-4 (IL-4), a potent anti-inflammatory cytokine, is barely produced by MSCs, and MSC therapy suffers from rapid MSC death following intra-articular implantation. MSCs in spheroids survive better than naïve MSCs in vitro and in vivo. IL-4-transfected MSCs in spheroids (IL-4 MSC spheroid) show increased chondroprotective and anti-inflammatory effects in an OA chondrocyte model in vitro. Following intra-articular implantation in OA rats, IL-4 MSC spheroids show better cartilage protection and pain relief than naïve MSCs. Thus, IL-4 MSC spheroid may potentiate the therapeutic efficacy of MSCs for OA.

中文翻译：

白细胞介素4基因转染和球体形成增强间充质干细胞治疗骨关节炎的疗效。

骨关节炎（OA）是一种痛苦而难治的疾病，严重影响患者的生活质量。但是，当前的疗法，例如止痛药和关节置换手术，并没有导致软骨保护。间充质干细胞（MSCs）已被提议作为OA治疗的替代策略，因为MSCs可以分泌软骨保护和抗炎因子。但是，MSC几乎不产生有效的抗炎细胞因子白细胞介素4（IL-4），并且在关节内植入后，MSC治疗会迅速死亡。球体中的MSC在体外和体内的存活率均优于未处理的MSC。IL-4转染的球体中的MSC（IL-4 MSC球体）在体外OA软骨细胞模型中显示出增强的软骨保护和抗炎作用。在OA大鼠中进行关节内植入后，IL-4 MSC球体比单纯MSC表现出更好的软骨保护和止痛效果。因此，IL-4 MSC球体可以增强MSC对OA的治疗功效。

更新日期：2020-03-04

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