Biolasing from Individual Cells in a Low‐Q Resonator Enables Spectral Fingerprinting,Advanced Optical Materials

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Biolasing from Individual Cells in a Low‐Q Resonator Enables Spectral Fingerprinting
Advanced Optical Materials ( IF 8.0 ) Pub Date : 2020-01-24 , DOI: 10.1002/adom.201901573
Dedy Septiadi ₁ , Valentin Barna _{2,

3} , Dhruv Saxena ₄ , Riccardo Sapienza ₄ , Damiano Genovese _{2,

5} , Luisa De Cola ₁

Affiliation

Lasing from cells has recently been subject of thorough investigation because of the potential for sensitive and fast biosensing. Yet, lasing from individual cells has been studied in high‐quality resonators, resulting in limited dependence of the lasing properties on the cellular microenvironment. Here, lasing is triggered by cells floating in a low quality factor resonator composed of a disposable poly(methyl methacrylate) (PMMA) cell counting‐slide, hence in absence of conventional high‐reflectivity optical cavities. The exceptional spectral narrowing and the steep slope increase in the input–output energy diagram prove occurrence of laser action in presence of cells. The observed biolasing is an intrinsically dynamic signal, with large fluctuations in intensity and spectrum determined by the optical properties of the individual cell passing through the pump beam. Numerical simulations of the scattering efficiency rule out the possibility of optical feedback from either WGM (whispering gallery mode) or multiple scattering within the cell, and point to the enhanced directional scattering field as the crucial contribution of cells to the laser action. Finally, principal component analysis of lasing spectra measured from freely diffusing cells yields spectral fingerprints of cell populations, which allows discriminating cancer from healthy Rattus glial cells with high degree of confidence.

中文翻译：

Low-Q谐振器中单个细胞的生物激射可实现光谱指纹识别

由于敏感和快速的生物传感的潜力，从细胞中释放激光已成为最近的研究重点。然而，已经在高质量谐振器中研究了单个细胞的发射，导致发射特性对细胞微环境的依赖性有限。在这里，激光发射是由漂浮在低质量因数谐振器中的细胞触发的，该谐振器由一次性聚甲基丙烯酸甲酯（PMMA）细胞计数滑块组成，因此没有传统的高反射率光学腔。输入-输出能量图中异常的光谱变窄和陡峭的斜率增加证明了存在细胞时激光作用的发生。观察到的生物激光是一种内在的动态信号，强度和光谱的大波动取决于通过泵浦光束的单个单元的光学特性。散射效率的数值模拟排除了细胞内WGM（回音壁模式）或多重散射产生光反馈的可能性，并指出增强的定向散射场是细胞对激光作用的关键贡献。最后，对从自由扩散细胞测得的激光光谱进行主成分分析，得出了细胞群体的光谱指纹图谱，从而可以高度自信地将癌症与健康的大鼠tus胶质细胞区分开。散射效率的数值模拟排除了细胞内WGM（回音壁模式）或多重散射产生光反馈的可能性，并指出增强的定向散射场是细胞对激光作用的关键贡献。最后，对从自由扩散细胞测得的激光光谱进行主成分分析，得出了细胞群体的光谱指纹图谱，从而可以高度自信地将癌症与健康的大鼠tus胶质细胞区分开。散射效率的数值模拟排除了细胞内WGM（回音壁模式）或多重散射产生光反馈的可能性，并指出增强的定向散射场是细胞对激光作用的关键贡献。最后，对从自由扩散细胞测得的激光光谱进行主成分分析，得出了细胞群体的光谱指纹图谱，从而可以高度自信地将癌症与健康的大鼠tus胶质细胞区分开。

更新日期：2020-03-20

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