In this work, we describe the complete sequence and genome organization of a novel tobamovirus detected in a prickly pear plant (Opuntia sp.) by high-throughput sequencing, tentatively named "opuntia virus 2". The full genome of opuntia virus 2 is 6,453 nucleotides in length and contains four open reading frames (ORFs) coding for the two subunits of the RNA polymerase, the movement protein, and the coat protein, respectively. Phylogenetic analysis using the complete nucleotide sequence revealed that the virus belongs to the genus Tobamovirus (family Virgaviridae), showing the highest nucleotide sequence identity (49.8%) with cactus mild mottle virus (CMMoV), being indicating that it belongs in the Cactaceae subgroup of tobamoviruses.

中文翻译：

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仙人掌果中一种新型烟草花叶病毒的鉴定和基因组鉴定。

在这项工作中，我们描述了通过高通量测序在花椒梨植物（Opuntia sp。）中检测到的新型烟草花叶病毒的完整序列和基因组结构，暂定名为“仙人掌病毒2”。仙人掌病毒2的完整基因组的长度为6,453个核苷酸，并包含四个开放阅读框（ORF），分别编码RNA聚合酶，运动蛋白和外壳蛋白的两个亚基。使用完整核苷酸序列进行的系统发育分析表明，该病毒属于Tobamovirus属（Virgaviridae家族），与仙人掌轻斑驳病毒（CMMoV）的核苷酸序列同一性最高（49.8％），表明该病毒属于Cactaceae亚组。烟草花叶病毒。