A cross-priming isothermal amplification (CPA) assay was developed for detection of feline herpesvirus type 1 (FHV-1). In this assay, the target fragment of the FHV-1 glycoprotein B gene is amplified rapidly by Bst DNA polymerase at a constant temperature (63 °C, 45 min), using a simple thermostat. The assay had no cross-reactions with four types of feline viruses, and the detection limit was 100 copies/μl. The positive rate of clinical samples from CPA was 100% consistent with qPCR but higher than ordinary PCR, indicating its superiority to ordinary PCR. Visualization was achieved using SYBR Green I dye.

中文翻译：

---

开发基于糖蛋白B基因的交叉引物等温扩增测定法，用于快速，快速检测1型猫疱疹病毒。

开发了一种交叉启​​动等温扩增（CPA）检测试剂盒，用于检测1型猫疱疹病毒（FHV-1）。在该测定中，使用简单的恒温器，通过Bst DNA聚合酶在恒定温度（63°C，45分钟）下快速扩增FHV-1糖蛋白B基因的目标片段。该测定与四种类型的猫病毒无交叉反应，检出限为100拷贝/μl。CPA临床样品的阳性率与qPCR一致，为100％，但高于普通PCR，表明其优于普通PCR。使用SYBR Green I染料实现了可视化。