Recent in vitro and in vivo studies on anthocyanins confirmed numerous health-promoting effects in humans. Daily anthocyanin intake can be estimated via food databases, but the amount absorbed by the organism still remains uncertain because anthocyanin bioavailability is yet to be elucidated in its entirety. For this purpose, suitable and validated methods of sample preparation and analysis are required. Therefore, a sample preparation method for anthocyanin metabolite analysis in plasma was successfully established and validated. The validation yielded acceptable results for the anthocyanins in terms of recovery (54–108%) and precision (coefficient of variation (CV) < 15%). The UHPLC-MS method used in the consecutive reaction monitoring (CRM) mode was sufficiently sensitive, resulting in limits of detection <2.3 ng/mL and limits of quantification < 8.1 ng/mL with associated repeatability of the MS system with CVs of <5.1%. In addition, a method for the sum parameter determination of anthocyanidins in urine comprising solely the evaporation of acidified samples was developed, validated, and successfully applied to real samples. The results showed that this method is applicable for the methylated anthocyanidins, but not for the hydroxylated anthocyanidins, due to the chosen CRM modes required for optimum selectivity.

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UHPLC-MSn 测定生理液体中花青素的方法的开发和验证

最近对花青素的体外和体内研究证实了许多对人类健康的促进作用。每日花青素摄入量可以通过食物数据库估算，但生物体吸收的量仍然不确定，因为花青素的生物利用度尚未完全阐明。为此，需要合适且经过验证的样品制备和分析方法。因此，成功建立并验证了一种用于血浆中花青素代谢物分析的样品制备方法。就回收率 (54–108%) 和精密度（变异系数 (CV) < 15%）而言，验证产生了可接受的花青素结果。连续反应监测 (CRM) 模式中使用的 UHPLC-MS 方法具有足够的灵敏度，导致检测限 <2。3 ng/mL 和定量限 < 8.1 ng/mL，具有相关联的 MS 系统重复性，CV <5.1%。此外，开发、验证并成功应用于实际样品中，仅包括酸化样品蒸发的尿液中花色素总和参数测定方法。结果表明，该方法适用于甲基化花色素，但不适用于羟基化花色素，因为选择了最佳选择性所需的 CRM 模式。