Prenylated stilbenoids are phenolic compounds produced in a small number of plants such as peanut (Arachis hypogaea) to counteract biotic and abiotic stresses. In addition to their role in plant defense, they exhibit biological activities with potential application in human health. Whereas non-prenylated stilbenoids such as resveratrol are commercially available, the availability of prenylated stilbenoids is limited. To this end, hairy root cultures of peanut were developed as an elicitor-controlled bioproduction platform for prenylated stilbenoids. An orthogonal array design approach led to the elucidation of an optimized elicitation procedure consisting of co-treatment of the hairy root cultures with 18 g/L methyl-β-cyclodextrin, 125 µM methyl jasmonate, 3 mM hydrogen peroxide (H2O2) and medium supplementation with additional 1 mM magnesium chloride. After 168-h of elicitor treatment, the combined yield of the prenylated stilbenoids arachidin-1, arachidin-2, arachidin-3 and arachidin-5 reached approximately 750 mg/L (equivalent to 107 mg/g DW). Moreover, hairy root cultures from the wild Arachis species A. duranensis and A. ipaensis were developed and shown to produce prenylated stilbenoids upon elicitor treatment. These wild Arachis hairy root lines may provide a platform to elucidate the biosynthetic origin of prenylated stilbenoids in peanut.

中文翻译：

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通过优化的诱导程序在花生 (Arachis hypogaea) 及其野生近缘 A. ipaensis 和 A. duranensis 的毛状根培养物中生产异戊二烯化芪类化合物

异戊二烯化芪是在少数植物中产生的酚类化合物，例如花生 (Arachis hypogaea)，用于抵消生物和非生物胁迫。除了在植物防御中的作用外，它们还表现出对人类健康具有潜在应用的生物活性。尽管非异戊二烯化芪类化合物如白藜芦醇可商购获得，但异戊二烯化芪类化合物的可用性有限。为此，花生的毛状根培养物被开发为异戊二烯化二苯乙烯的诱导子控制的生物生产平台。正交阵列设计方法阐明了优化的诱导程序，包括用 18 g/L 甲基-β-环糊精、125 µM 茉莉酸甲酯、3 mM 过氧化氢 (H2O2) 和额外添加 1 mM 氯化镁的培养基。诱导剂处理 168 小时后，异戊二烯化芪类化合物 arachidin-1、arachidin-2、arachidin-3 和 arachidin-5 的总产量达到约 750 mg/L（相当于 107 mg/g DW）。此外，开发了来自野生花生属物种 A. duranensis 和 A. ipaensis 的毛状根培养物，并显示在诱导剂处理后产生异戊二烯化芪类化合物。这些野生花生毛状根系可为阐明花生中异戊二烯化二苯乙烯的生物合成来源提供一个平台。开发了来自野生花生种 A. duranensis 和 A. ipaensis 的毛状根培养物，并显示在诱导剂处理后产生异戊二烯化芪类。这些野生花生毛状根系可为阐明花生中异戊二烯化二苯乙烯的生物合成来源提供一个平台。开发了来自野生花生种 A. duranensis 和 A. ipaensis 的毛状根培养物，并显示在诱导剂处理后产生异戊二烯化芪类化合物。这些野生花生毛状根系可为阐明花生中异戊二烯化二苯乙烯的生物合成来源提供一个平台。