Abstract

A new strategy for the surface modification of bacterial cellulose (BC) through the combination of oxygen plasma deposition and silanization with trichloromethyl silane (TCMS) is described. The combined use of the two techniques modifies both the surface roughness and energy and therefore maximizes the obtained hydrophobic effect. These modified membranes were characterized by Scanning Electron Microscopy (SEM), water contact angle measurements, Fourier-transform infrared spectroscopy (FTIR-ATR) and X-ray photoelectron spectroscopy (XPS), and its cytotoxic potential was investigated using both indirect and direct contact in vitro studies. The obtained results suggest an effective conjugation of TCMS to the surface of BC, leading to a highly hydrophobic surface, with a water contact angle of approximately 130º. It is also demonstrated that this is a stable and durable surface modification strategy, since BC remained hydrophobic even after 6 months, in dry conditions or after being submerged in distilled water for about a month. Importantly, this surface modification revealed no short-term cytotoxic effects on L929 and hDNFs cells. Altogether, these data indicate the successful development of a surface modification method that can be applied to BC, enabling the production of a biodegradable and hydrophobic platform that can be applied to different areas of research and industry.

Graphic abstract

中文翻译：

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使用氧等离子体处理和化学气相沉积对细菌纤维素进行疏水改性

摘要

描述了一种通过氧等离子体沉积和硅烷化与三氯甲基硅烷（TCMS）相结合的细菌纤维素（BC）表面改性的新策略。两种技术的组合使用可同时改变表面粗糙度和能量，因此可最大程度地获得疏水效果。这些修饰的膜通过扫描电子显微镜（SEM），水接触角测量，傅立叶变换红外光谱（FTIR-ATR）和X射线光电子能谱（XPS）进行了表征，并通过间接和直接接触研究了其细胞毒性潜力。体外研究。获得的结果表明，TCMS与BC表面有效结合，形成高度疏水的表面，水接触角约为130º。还证明了这是一种稳定且持久的表面改性策略，因为即使在6个月后，在干燥条件下或浸入蒸馏水中约一个月后，BC仍保持疏水性。重要的是，这种表面修饰显示对L929和hDNFs细胞没有短期细胞毒性作用。总而言之，这些数据表明可以应用于BC的表面改性方法的成功开发，从而能够生产可应用于不同研究和工业领域的可生物降解和疏水性平台。这种表面修饰显示对L929和hDNFs细胞没有短期细胞毒性作用。总而言之，这些数据表明可以应用于BC的表面改性方法的成功开发，从而能够生产可应用于不同研究和工业领域的可生物降解和疏水性平台。这种表面修饰显示对L929和hDNFs细胞没有短期细胞毒性作用。总而言之，这些数据表明可以应用于BC的表面改性方法的成功开发，从而能够生产可应用于不同研究和工业领域的可生物降解和疏水性平台。

图形摘要