Biosynthetic Mycotoxin Conjugate Mimetics-Mediated Green Strategy for Multiplex Mycotoxin Immunochromatographic Assay.,Journal of Agricultural and Food Chemistry

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Biosynthetic Mycotoxin Conjugate Mimetics-Mediated Green Strategy for Multiplex Mycotoxin Immunochromatographic Assay.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-24 , DOI: 10.1021/acs.jafc.9b06383
Jia-Xiang Yan _{1,

2,

3} , Wen-Jin Hu _{1,

2,

3} , Kai-Hao You _{1,

2,

3} , Zhen-E Ma _{1,

2,

3} , Yang Xu _{1,

2,

3} , Yan-Ping Li _{1,

2,

3} , Qing-Hua He _{1,

2,

3,

4}

Affiliation

Various mycotoxins widely co-exist in agro-products, and their combined effects cause toxicity and potential carcinogenicity to humans and animals. In this work, we developed an economical and sensitive quantum dots (QDs)/QD microbead (QDs/QB)-based multiplex immunochromatographic assay (mICA) for the rapid detection of fumonisin B1 (FB1), zearalenone (ZEN), and ochratoxin A (OTA) without the building-up process of mycotoxin conjugates. QDs and QBs were selected as fluorescent reporters and conjugated with antimycotoxin monoclonal antibodies for improving sensitivity. Furthermore, phage-displayed FB1, ZEN, and OTA mimotope peptide-based soluble and monovalent fusions to maltose-binding protein (MBP) were applied onto the test line of the mICA as the mimetic coating antigen. Under the optimized conditions, the visual detection limits (vLODs) of peptide-MBP-based mICA could be obtained as 0.25 ng/mL for FB1, 3.0 ng/mL for ZEN, and 0.5 ng/mL for OTA within 10 min. The results for spiked real sample detection indicate good accuracy, reproducibility, and practicability. In addition, the proposed mICA was comparable with ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) in terms of reliability in detecting FB1, ZEN, and OTA using natural samples. From the point of promoting commercial production, these time-saving and low-cost peptide-MBP antigens applied in ICA might provide promising potential for promoting productivity and decreasing the cost of production.

中文翻译：

生物合成霉菌毒素结合模拟物介导的绿色策略，用于多重霉菌毒素免疫色谱分析。

各种真菌毒素广泛共存于农产品中，它们的综合作用对人类和动物造成毒性和潜在的致癌性。在这项工作中，我们开发了一种经济且敏感的基于量子点（QD）/ QD微珠（QDs / QB）的多重免疫色谱分析（mICA），用于快速检测伏马菌素B1（FB1），玉米赤霉烯酮（ZEN）和曲霉毒素A （OTA），无需建立霉菌毒素偶联物。选择QD和QB作为荧光报告基因，并与抗霉菌毒素单克隆抗体偶联以提高敏感性。此外，将噬菌体展示的FB1，ZEN和OTA模拟表位肽与麦芽糖结合蛋白（MBP）的可溶性和单价融合物作为模拟的包被抗原应用于mICA的测试线。在优化条件下在10分钟内，基于肽-MBP的mICA的视觉检测限（vLOD）可以分别为FB1 0.25 ng / mL，ZEN 3.0 ng / mL和OTA 0.5 ng / mL。加标真实样品检测的结果表明良好的准确性，可重复性和实用性。此外，在使用天然样品检测FB1，ZEN和OTA的可靠性方面，拟议的mICA可与串联质谱联用的超高效液相色谱（UPLC-MS / MS）相提并论。从促进商业生产的角度来看，ICA中使用的这些省时且低成本的多肽MBP抗原可能为提高生产率和降低生产成本提供有希望的潜力。加标真实样品检测的结果表明良好的准确性，可重复性和实用性。此外，在使用天然样品检测FB1，ZEN和OTA的可靠性方面，拟议的mICA可与串联质谱联用的超高效液相色谱（UPLC-MS / MS）相提并论。从促进商业生产的角度来看，ICA中使用的这些省时且低成本的多肽MBP抗原可能为提高生产率和降低生产成本提供有希望的潜力。加标真实样品检测的结果表明良好的准确性，可重复性和实用性。此外，在使用天然样品检测FB1，ZEN和OTA的可靠性方面，拟议的mICA可与串联质谱联用的超高效液相色谱（UPLC-MS / MS）相提并论。从促进商业生产的角度来看，ICA中使用的这些省时且低成本的多肽MBP抗原可能为提高生产率和降低生产成本提供有希望的潜力。

更新日期：2020-02-06

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