An electric soldering iron ion source (ESII) coupling with rapid evaporative ionization mass spectrometry (REIMS) was developed and used for in situ monitoring the dynamic variation trend in oxidation characteristics of fish oil during storage. The lipidomics profiles of fish oil stored at various days were acquired by ESII-REIMS. The fatty acid and triacylglycerol species were structurally identified, and their abundances were analyzed according to multivariate statistical models mainly including principle component analysis as well as orthogonal partial least-squares analysis. On the shared and unique structure plot, the ions of m/z 255.23, 281.24, 877.72, and 901.72 displayed the most significant variation among the oxidized fish oil samples. Based on receiver operating characteristic curve analysis with an optimal Youden index of 0.91, these markers were further verified. The variation of viscosity and volatiles were also evaluated to further verify the oxidation characteristics of fish oil. The study demonstrated that ESII-REIMS technology used as an advanced detection method could ensure fish oil quality during storage.

中文翻译：

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基于电烙铁电离质谱的脂质组学，用于在存储过程中原位监测鱼油的氧化特性。

开发了与快速蒸发电离质谱（REIMS）耦合的电烙铁离子源（ESII），并用于现场监测鱼油在存储过程中氧化特性的动态变化趋势。通过ESII-REIMS获得了在不同日期存储的鱼油的脂质组学概况。通过多变量统计模型，主要包括主成分分析和正交偏最小二乘分析，对脂肪酸和三酰基甘油进行结构鉴定，并分析其丰度。在共享的唯一结构图上，m / z 255.23、281.24、877.72和901.72的离子在氧化的鱼油样品中显示出最大的变化。根据最佳Youden指数为0.91的接收机工作特性曲线分析，这些标记物进一步得到验证。还评估了粘度和挥发物的变化，以进一步验证鱼油的氧化特性。研究表明，ESII-REIMS技术作为一种先进的检测方法可以确保储存过程中鱼油的质量。