One-step synthesis of rhodamine-based Fe3+ fluorescent probes via Mannich reaction and its application in living cell imaging.,Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

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One-step synthesis of rhodamine-based Fe3+ fluorescent probes via Mannich reaction and its application in living cell imaging.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.saa.2020.118105
Mengyao Zhang ₁ , Chuanchuan Shen ₁ , Ting Jia ₁ , Jianwen Qiu ₁ , Hu Zhu ₂ , Yong Gao ₂

Affiliation

Four rhodamine-based fluorescent probes M1-M4 were synthesized in one step using Mannich reaction. The Mannich reaction based approach has the advantages of simplicity, good yield and excellent atomic economy. The structures were determined by 1H NMR, 13C NMR, IR and HRMS. The probe M3 as a representative compound was characterized by single-crystal X-ray analyses. The fluorescence and absorbance spectra research of the probes demonstrated that they could be used as Fe3+-selective fluorescent probes with good sensitivity, excellent linearity, and outstanding anti-interference in acetonitrile/Tris-HCl buffer solution (3:7, V/V; pH = 7.4). Moreover, confocal laser scanning microscopy experiments have proven that the probe M3 was successfully used for fluorescence imaging in MCF-7 cells.

中文翻译：

通过曼尼希反应一步法合成若丹明基Fe3 +荧光探针及其在活细胞成像中的应用。

使用曼尼希反应一步一步合成了四个基于罗丹明的荧光探针M1-M4。基于曼尼希反应的方法具有简单，产率高和原子经济性好的优点。结构通过1 H NMR，13 C NMR，IR和HRMS确定。通过单晶X射线分析表征作为代表性化合物的探针M3。探针的荧光和吸收光谱研究表明，它们可以用作Fe3 +选择性荧光探针，灵敏度高，线性好且在乙腈/ Tris-HCl缓冲溶液中具有出色的抗干扰性（3：7，V / V; pH = 7.4）。此外，共聚焦激光扫描显微镜实验已经证明，探针M3已成功用于MCF-7细胞的荧光成像。

更新日期：2020-01-24

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