Oxyeleotris marmoratus iridovirus (OMIV) and Oxyeleotris marmoratus rhabdovirus (OMRV) are the two major causative agents of disease leading to massive mortality and severe economic losses in marbled sleepy goby (Oxyeleotris marmoratus) industry. It's urgent to develop an effective vaccine against these fatal diseases. In this study, we developed bivalent inactivated vaccine against OMIV and OMRV and evaluated its protective effect in Oxyeleotris marmoratus. The intraperitoneally vaccinated fish were protected against challenge with OMIV and OMRV with both relative percent survival (RPS) of 100%. In addition, deep RNA sequencing was used to analyze the transcriptomic profiles of the spleen tissues at progressive time points post-vaccination with bivalent inactivated vaccine and challenge with OMIV and OMRV infection. Results showed that adaptive immune response was induced in Oxyeleotris marmoratus injected with bivalent inactivated vaccine. Furthermore, robust adaptive immune responses were also detected in vaccinated fish at 7 d and 2 d post-challenge with OMIV and OMRV. Taken together, these results indicated that bivalent inactivated vaccine activated adaptive immune responses in Oxyeleotris marmoratus, and provided protection against OMIV and OMRV lethal challenge.

中文翻译：

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转录组谱显示，灭活的虹膜病毒和弹状病毒二价疫苗引发了强大的适应性免疫反应，可抵抗大理石困的虾虎鱼的致死性攻击。

早疫莫氏弧菌病毒（OMIV）和早疫莫氏弧菌病毒（OMRV）是导致该病的两个主要病原体，导致大理石状昏昏的虾虎鱼（Oxyeleotris marmoratus）行业的大量死亡和严重经济损失。迫切需要开发出一种针对这些致命疾病的有效疫苗。在这项研究中，我们开发了针对OMIV和OMRV的二价灭活疫苗，并评估了其对早疫病菌（Oxyeleotris marmoratus）的保护作用。腹腔内接种的鱼可以相对抵抗存活率（RPS）为100％的OMIV和OMRV攻击。另外，深度RNA测序被用于分析在接种二价灭活疫苗并受到OMIV和OMRV感染的攻击后的渐进时间点的脾组织的转录组谱。结果表明，注射二价灭活疫苗后，在Oxyeleotris marmoratus中诱导了适应性免疫反应。此外，在用OMIV和OMRV攻击后7天和2天，在接种疫苗的鱼中也检测到了强大的适应性免疫反应。综上所述，这些结果表明，二价灭活疫苗激活了氧化Ox（Oxyeleotris marmoratus）中的适应性免疫反应，并提供了针对OMIV和OMRV致死性攻击的保护作用。