The large-scale untargeted proteomic and metabolomic studies were conducted in strawberry (Fragaria × ananassa) cv. Akihime fruit at five developmental stages. We found that some C6 volatiles highly contributed to the enrichment of volatiles at the red stage of strawberry fruit. We found that 12 genes involved in LOX pathway for volatile biosynthesis showed multiple patterns in protein abundance during fruit development and ripening, and 9 out of the 12 genes exhibited a significant increase in their relative expression levels at the red stage of fruit. We also found that the MYB9 gene (FaMYB9) expression level was positively correlated with the content of C6 volatiles (R = 0.989) and with the relative expression level and protein abundance of FaLOX5 at different strawberry fruit developmental stages (R = 0.954). The interaction between FaMYB9 and FaLOX5 was detected by yeast two-hybrid, co-immunoprecipitation (Co-IP), bimolecular fluorescence complementation (BiFC), and immunofluorescence (IF) analyses. Transient silencing of FaMYB9 delayed the fruit development and ripening, resulting in a significant decrease in the contents of C6 volatiles, while overexpression of FaMYB9 increased the fruit development and ripening and the contents of C6 volatiles in Akihime fruit. Therefore, FaMYB9 is positively involved in C6 volatile biosynthesis.

中文翻译：

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FaMYB9参与草莓C6挥发性生物合成的调控

在草莓 (Fragaria × ananassa) cv. 中进行了大规模的非靶向蛋白质组学和代谢组学研究。五个发育阶段的 Akihime 果实。我们发现一些C6挥发物对草莓果实红色期挥发物的富集有很大贡献。我们发现，参与挥发性生物合成 LOX 途径的 12 个基因在果实发育和成熟过程中表现出多种蛋白质丰度模式，12 个基因中有 9 个在果实红色阶段表现出相对表达水平的显着增加。我们还发现MYB9基因（FaMYB9）的表达水平与C6挥发物的含量（R = 0.989）以及不同草莓果实发育阶段FaLOX5的相对表达水平和蛋白质丰度（R = 0.954）呈正相关。FaMYB9 和 FaLOX5 之间的相互作用通过酵母双杂交、免疫共沉淀 (Co-IP)、双分子荧光互补 (BiFC) 和免疫荧光 (IF) 分析检测到。FaMYB9的瞬时沉默延迟了果实发育和成熟，导致C6挥发物含量显着降低，而FaMYB9的过表达增加了果实发育和成熟以及Akihime果实中C6挥发物的含量。因此，FaMYB9 积极参与 C6 挥发性生物合成。而 FaMYB9 的过表达增加了 Akihime 果实的发育和成熟以及 C6 挥发物的含量。因此，FaMYB9 积极参与 C6 挥发性生物合成。而 FaMYB9 的过表达增加了 Akihime 果实的发育和成熟以及 C6 挥发物的含量。因此，FaMYB9 积极参与 C6 挥发性生物合成。