In the present study, we investigated the neuroprotective effects of a purified Corydalis yanhusuo polysaccharide (CYP) on Aβ (25-35)-induced neurotoxicity and explored its underlying molecular mechanisms in PC12 cells. The results showed pretreatment with CYP (25, 50, and 100 μg/ml) prior to Aβ (25-35) exposure significantly protected PC12 cells from Aβ (25-35)-induced cell death, lactate dehydrogenase (LDH) release, DNA fragmentation, mitochondrial dysfunction and mitochondrial cytochrome c release. Moreover, Aβ (25-35)-induced increase of ratio between Bax and Bcl-2 protein expression was dramatically reversed by CYP pretreatment. Furthermore, the addition of CYP led to a significant repressing effect on the elevated protein expression of cleaved caspase-8, caspase-9, and caspase-3 in Aβ (25-35)-treated PC12 cells. Taken together, these findings indicated that protective effect of CYP against Aβ (25-35)-induced toxicity in PC12 cells was probably mediated by inhibition of apoptosis via both mitochondrial apoptotic pathway and death receptor pathway.

中文翻译：

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延胡索多糖的结构表征和神经保护作用。

在本研究中，我们研究了纯化的延胡索多糖（CYP）对Aβ（25-35）诱导的神经毒性的神经保护作用，并探讨了其在PC12细胞中的潜在分子机制。结果显示，在暴露于Aβ（25-35）之前，先用CYP（25、50和100μg/ ml）预处理可显着保护PC12细胞免受Aβ（25-35）诱导的细胞死亡，乳酸脱氢酶（LDH）释放，DNA碎裂，线粒体功能障碍和线粒体细胞色素c释放。此外，CYP预处理可显着逆转Aβ（25-35）诱导的Bax和Bcl-2蛋白表达比率的增加。此外，CYP的添加导致对经Aβ（25-35）处理的PC12细胞中裂解的caspase-8，caspase-9和caspase-3蛋白表达升高的显着抑制作用。在一起