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Enzyme embedded metal organic framework (enzyme–MOF): De novo approaches for immobilization
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2020-01-24 , DOI: 10.1016/j.ijbiomac.2020.01.240
Shamraja S. Nadar , Leena Vaidya , Virendra K. Rathod

The porous material has been considered as a potential candidate for immobilizing enzymes. Recently, metal organic framework (MOF) has been emerged as a hybrid organic inorganic material with unique intrinsic properties such as well-defined pore structure, excellent chemico-thermal stability, and extremely high surface areas which make them as a suitable scaffold for enzyme immobilization. The outstanding improvement in catalytic performance, high enzyme loading capacity, remarkable interaction between enzyme and MOF are the key features of the novel enzyme–MOF biocomposites. Amongst different immobilization approaches of enzyme–MOF composite development, de novo strategy received immense attention due to rapid, facile, mild immobilization procedure which exhibits potentially superior catalytic activity and extraordinary operational stability. This review presents a holistic insight of two different de novo strategies i.e. co-precipitation and biomineralization with state-of-art examples. Further, the recent developments in enzyme–MOF composites along with their potential features and characteristics are exploited in terms of catalytic activity, thermal/chemical stability, Michaelis–Menten kinetics, recyclability and storage stability. The advanced de novo strategies such as multi-enzyme catalytic system and magnetic enzyme–MOF are explored in the latter part of highlights.



中文翻译:

酶嵌入的金属有机框架(酶– MOF):从头开始的固定方法

多孔材料被认为是固定化酶的潜在候选者。近年来,金属有机骨架(MOF)已作为具有独特内在特性的杂化有机无机材料出现,如明确的孔结构,优异的化学热稳定性和极高的表面积,使其成为酶固定化的合适支架。 。催化性能的显着提高,高酶负载能力,酶与MOF之间的显着相互作用是新型酶MOF生物复合材料的关键特征。从头开始进行酶-MOF复合材料开发的不同固定方法由于快速,简便,温和的固定程序具有潜在的优越的催化活性和非凡的操作稳定性,因此该策略受到了广泛的关注。本文综述了两种不同的整体洞察从头策略,即共沉淀和生物矿化与国家的最先进的例子。此外,在催化活性,热/化学稳定性,Michaelis-Menten动力学,可回收性和储存稳定性方面,还利用了酶-MOF复合材料的最新发展及其潜在的特征和特性。后期重点探讨了先进的从头策略,例如多酶催化系统和磁性酶-MOF。

更新日期:2020-01-24
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